Figure 2 (IMAGE) Institute for Basic Science Caption PAM compatibilities for SpCas9 variants. (a) Darker colors indicates higher frequency of DNA cleavage. (b) Among these four variants (SpCas9, VRQR, xCas9 and SpCas9-NG), SpCas9-NG has been the traditional choice for all PAM sequences that have a guanine (G) as the second nucleotide. However, these results shows that for PAM sequences AGAG and GGCG, for example, the Cas9 variant VRQR (in blue) would be preferable. Credit IBS Usage Restrictions Please indicate the credit. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.