Genetic and Metabolic Analyses Reveal the D-Stereospecific Hydrolytic Activities of DRPs (IMAGE)

Hong Kong University of Science and Technology

Genetic and Metabolic Analyses Reveal the D-Stereospecific Hydrolytic Activities of DRPs

Caption

a, The BGCs of bogorol from B. laterosporus DSM 25 and ATCC 9141, as well as tridecaptin A and tridecaptin B from P. polymyxa CICC 10580. BogQ from the strain DSM 25 and that from the strain ATCC 9141 share 85 percent amino acid sequence identity. The intersecting dotted lines indicate genes shared by two gene clusters within the same host (Supplementary Fig. 10). b, Structures of DNRPs tridecaptin B and bogorol A, with DRP recognition motifs highlighted. c, Stacked overlay of the mass spectra (electrospray ionization) of parent compounds (black), C-terminal fragments (blue), and N-terminal fragments (red). Data are representative of two independent experiments. Top, tridecaptin B; bottom, bogorol A. d, Time-course analyses of corresponding compounds produced by P. polymyxa CICC 10580 (top) and B. laterosporus DSM 25 (bottom) at different fermentation times (representative of three independent experiments).

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Division of Life Science, HKUST

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