Biochemical Analyses Reveal the Broad-Spectrum Resistance of BogQ against Peptide Antibiotics. (IMAGE)

Hong Kong University of Science and Technology

Biochemical Analyses Reveal the Broad-Spectrum Resistance of BogQ against Peptide Antibiotics.

Caption

a, LC-MS traces comparing wild-type B. laterosporus ATCC 9141 and the ΔbogQ mutant (representative of three independent experiments). b,c, Kinetic analyses of BogQ-catalyzed hydrolysis of bogorol A (b; 3) and bacitracin (c; 46) v, reaction velocit. Data are mean?±?s.d.; n=?3 independent experiments. d, Structure of the DNRP antibiotic bacitracin; colors highlight the cleavage sites of BogQ. e, LC-MS traces of in vitro assays of BogQ (2.0?μM) against 46 (200?μM; representative of two independent experiments). Time-course cleavage products (47-53) of 46 are labeled using the same color code as their d-aa cleavage sites in d. For enzymatic cleavage patterns, see Supplementary Fig. 22.

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Division of Life Science, HKUST

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