Figure 3. CCA1 Clock Protein Binds Near the PRR5 Gene within the Cell (IMAGE)
Caption
1) The CCA1 protein and chromosomal DNA are bound together within the plant cell; 2) Fragmentation of chromosomal DNA; 3) The CCA1 protein is collected and the DNA bound to is analyzed by rapid DNA sequencing; 4) The lead (DNA sequence) is mapped to the genome sequence to reveal the genome region that CCA1 binds to. (A) shows the actual and predicted positions that CCA1 binds to on the DNA sequence. CCA1 binds to three positions near the PRR5 gene. (B) The CCA1 protein represses the PRR5 gene during sunrise. As time proceeds, the amount of CCA1 decreases and the PRR5 gene is transcribed, leading to an increase in the PRR5 protein, which is responsible for various biological activities for the plant during the evening.
Credit
ITbM, Nagoya University
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