"Ultra-rapid detection of SARS-CoV-2 in public workspace environments (IMAGE)
Caption
Displaceable probe LAMP to detect SARS-CoV-2. (A) Classical RT-LAMP utilizes six primers hybridizing to eight regions within the viral genome. These primers form a dumbbell structure through self-hybridization of FIP and BIP and addition of two loop primers improves the amplification rate. To allow simultaneous detection of several targets in real-time, displaceable probe architecture was employed by tagging one of the loop primers with a quencher and supplementing with a partially complementary probe containing a fluorophore tag. (B) Positive results can be analyzed in real-time and process manifests itself as sigmoidal curve as it would be in RT-qPCR using TaqMan probes. (C) End-point fluorescence is then observed through orange filter coupled with blue LED (exc. 470 nm, Firebird Biomolecular Sciences LLC, US).
Credit
Yaren et al, PLOS ONE, 2021 (CC-BY 4.0, https://creativecommons.org/licenses/by/4.0/)
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