image: Displaceable probe LAMP to detect SARS-CoV-2. (A) Classical RT-LAMP utilizes six primers hybridizing to eight regions within the viral genome. These primers form a dumbbell structure through self-hybridization of FIP and BIP and addition of two loop primers improves the amplification rate. To allow simultaneous detection of several targets in real-time, displaceable probe architecture was employed by tagging one of the loop primers with a quencher and supplementing with a partially complementary probe containing a fluorophore tag. (B) Positive results can be analyzed in real-time and process manifests itself as sigmoidal curve as it would be in RT-qPCR using TaqMan probes. (C) End-point fluorescence is then observed through orange filter coupled with blue LED (exc. 470 nm, Firebird Biomolecular Sciences LLC, US). view more
Credit: Yaren et al, PLOS ONE, 2021 (CC-BY 4.0, https://creativecommons.org/licenses/by/4.0/)
Ultra-fast, cheap LAMP-based COVID tests could be performed by non-experts at work and in public spaces, giving results in under an hour
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Article Title: "Ultra-rapid detection of SARS-CoV-2 in public workspace environments"
Funding: This project was made possible through the support of a grant from Dynamic Combinatorial Chemistry, LLC, The funder provided support in the form of salaries and supplies for authors OY, ZY, JM, and BO, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section. The opinions expressed in this publication are those of the author(s) and do not necessarily reflect the views of DCC. Firebird Biomolecular Sciences, LLC, GenePath Diagnostics, Inc., and GenePath Diagnostics India Pvt. Ltd. employ the indicated authors and the specific roles of these authors are articulated in the 'author contributions' section.
Competing Interests: Firebird Biomolecular Sciences, LLC, GenePath Diagnostics, Inc., and GenePath Diagnostics India Pvt. Ltd. employ the indicated authors and the specific roles of these authors are articulated in the 'author contributions' section. OY, ZY, SAB and their institutions own intellectual property associated with the assay. Some of the items mentioned here are sold by Firebird Biomolecular Sciences, LLC, which employs the indicated authors and is owned by SAB. This does not alter the authors' and institutions' adherence to PLOS ONE policies on sharing data and materials.
Article URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0240524
Journal
PLoS ONE