Detection of GluR2 mRNA-positive Cells in Primary Hippocampal Cultures (IMAGE)

Lobachevsky University

Detection of GluR2 mRNA-positive Cells in Primary Hippocampal Cultures

Caption

(a-d) Representative confocal microscopy images of primary hippocampal cultures during normoxia (a - Sham; b - 72?h after GDNF (1?ng/ml) addition) and on the 3rd day of the post-hypoxic period (c - Hypoxia; d - Hypoxia+GDNF (1 ng/ml)). (A) GluR2 mRNA-positive cells in primary hippocampal culture; (B) primary hippocampal cells stained by Oregon Green 488 BAPTA-1 AM; (C) merged images in A and B. Oregon Green application allows visualization of living metabolically active cells in neural networks because the necessary condition for this dye to fluoresce is the presence of esterases (active enzymes). Combined staining with Oregon Green and RNA detection probes can identify the presence of mRNA in specific cell compartments (cell body or outgrowth). The confocal microscopy images show GluR2 mRNA localization predominantly in the soma and in separate regions of neuronal outgrowth. (A-C) Scale bar: 50?μm. (e) Percentage of GluR2 mRNA-positive cells. * versus "Sham"; # versus "Hypoxia", p?

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Lobachevsky University

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