Quantitative Analyses of Synapses in Wild-Type and NMDAR-Deficient Neurons (IMAGE)

RIKEN

Quantitative Analyses of Synapses in Wild-Type and NMDAR-Deficient Neurons

Caption

L5 neurons were labeled with cytoplasmic tdTomato and EYFP-gephyrin using in utero electroporation and analyzed at P22 (Airyscan). Unbranched oblique dendrites (100-200 μm long) extending from the trunk of the proximal part of the apical dendrites from L5 pyramidal neurons were analyzed. Representative super-resolution images are reconstructed with VAST. Note that gephryin puncta on the dendrite shaft vs. spines are unambiguously defined. Both wild-type (A) and NR1-deficient neurons (B) were analyzed.

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RIKEN

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