A Revolutionary Way to Map the Brain (IMAGE)

Cold Spring Harbor Laboratory

A Revolutionary Way to Map the Brain

Caption

An injection into a 'source' region of the brain contains a viral library encoding a diverse collection of barcode sequences, which are hitched to an engineered protein that is designed to carry the barcode along axonal pathways. The barcode RNA is expressed at high levels and transported into the terminals of axons in the source region where the injection is made. In each neuron, it travels to the point where the axon forms a synapse with a projection from another neuron.

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Zador Lab CSHL

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