Fig. 3 (IMAGE) Osaka University Caption Schematic representation of free energies before (a) and after (b) the linkage of folding and misfolding transitions. (a) A two-state transition independent of protein concentration persists. The high free energy barrier of supersaturation and the folding/unfolding transition between native (N) and unfolded (D) states is consistent with Anfinsen's dogma. (b) Upon breaking supersaturation, by agitation, the folding/unfolding transition and the aggregation to polymeric states (P) enter an equilibrium, and the solubility of D determines the overall equilibrium. Credit Osaka University Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.