Figure 3. (IMAGE) Science China Press Caption 3D spatial-frequency-shift tunable nanoscopy. (a) Spatial distribution of the 3D object.The colorbar represents the depth information. (b) The emission intensity distributions under the 30 different excitation intensities that are used for sectional imaging. (c) Reconstructed superresolution images at three layers with a depth of 7 nm, 14 nm, and 21 nm (noise-free). (d-e) Reconstructed 3D superresolution image with Poisson noise added. The peak photon number of the raw images is set as 106 (d) and 102 (e), respectively. (f) The true depth and reconstructed depth of the line-like object pointed by arrow I in (d-e). (g-h) The deviation of the reconstructed depth from the true object depth (?z), corresponding to the 3D reconstruction results shown in (d-e). (i) Intensity profiles corresponding to the position pointed by arrow II in (d-e). NA = 0.9; neff. = 3.38; λillu. = 561 nm; λemis. = 600 nm. Credit ©Science China Press Usage Restrictions Use with credit. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.