Figure 1: Dysregulated Chemokine Expression is Associated with Diminished T Cell Homing to Aged LNs (IMAGE)

Institute for Basic Science

Figure 1: Dysregulated Chemokine Expression is Associated with Diminished T Cell Homing to Aged LNs

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(A) Lymphocytes were isolated from B6.CD90.1+ Foxp3GFP+donor mice and 8.0 × 106 cells were injected into young (2 month) and aged (16 month) hosts. Bars show naïve donor T cell recovery in the pLN, mLN, and spleen of young and aged mice 4 hours after adoptive transfer. (B) Representative images are shown from young and aged pLN sections that were stained with the indicated antibodies and analyzed using immunofluorescence microscopy. Scale bars equal 50 μ m. (C) qPCR was used to determine the average normalized mRNA expression (± SD) of CCL21, CXCL13, and CCL19 in the pLN, mLN, and spleen of young and aged mice.

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