Figure 2: The Stromal Network is Altered in the LN of Aged Mice (IMAGE)
Caption
(A) LNs were removed from young and aged mice and FRCs were isolated by collagenase digestion. Plots show the gating strategy used to determine the total number of gp38+CD31?CD35? CD45? FRCs. Graph depicts the average number of FRCs recovered from the pLN of young and aged mice. (B) Representative images are shown from young and aged spleen sections stained with anti-gp38 and CD35 antibodies and analyzed by immunofluorescence microscopy. Scale bars equal 50 μm. (C) LN cells from young (2 month) or aged (20 month) mice were labeled with CMTPX (red) or CMTMR (blue), respectively, and 5.0 × 106 total cells were injected into age-matched littermate hosts. Hosts were injected with FITC-labeled anti-gp38 antibody 15-18 hours before analysis. Inguinal LN were explanted and visualized by multi-photon excitation microscopy. Images show representative data from 4 independent experiments and scale bars equal 50 μ m. (D) Naive T cells were labeled with CFSE and transferred into young and aged hosts. Representative images are shown from young and aged pLN vibratome sections stained with anti-gp38 and laminin antibodies analyzed by immunofluorescence microscopy. Scale bars are equal to 20 μ m.
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