Figure 1: CRCT forming a complex with 14-3-3 protein in the nucleus. (IMAGE)
Caption
Green fluorescent protein (GFP) was split into the N terminal and C terminal regions. Top row: Fluorescence from the reconstituted GFP was detected when the N terminal region was fused to CRCT and the C terminal region was fused to 14-3-3 protein, and these fusion proteins were expressed in the plant cell, indicating that CRCT can interact with 14-3-3 protein. DsRed is a red fluorescent protein that was used to confirm the genetic transformation. By staining the nucleus fluorescent blue with DAPI, it was possible to observe that the interaction of CRCT and 14-3-3 proteins took place in the nucleus. Middle row: On the other hand, GFP was not detected when CRCT was fused to both termini because CRCT proteins do not bind to each other. Bottom row: When both termini were fused with 14-3-3 proteins, the presence of GFP in the nucleus and cytoplasm indicated that 14-3-3 proteins bind to each other.
Credit
Modified Fig. 9 of Fukayama et al. <em>Plant, Cell & Environment </em>(2021)
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