Big Results from Small Solutions: New Method for Analyzing Metalloproteins (Figure 2) (IMAGE)
Caption
Results of the EPR measurements obtained in this study from a frozen solution sample. The top two diagrams are for hemin chloride, and the graph at the bottom is for myoglobin. The red line is a projected signal from a value simulator. The solution concentrations and sample volumes were 50 mM?2 μL for hemin chloride, and 8.8 mM?10 μL for myoglobin. Measurements took place at 4.2 K. By examining the two lines in the upper right graph we are able to determine the exact state of iron ions. The upper left graph shows the molecular structure of hemin chloride.
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Kobe University
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