Multi-mode imaging of urinary system and blood vessels under the excitation of a single 915 nm CW laser (IMAGE)
Caption
a, The optical principle of multi-mode imaging. 915 nm is the excitation wavelength for ICG to produce ASF, and is also the absorption peak of L1057 NPs, and the ASF of ICG in channel 1 is exactly located in the high absorption spectral region of L1057 NPs. Meanwhile Stokes fluorescence (SF) signals of L1057 NPs in channel 2 do not have crosstalk with that of ICG. b, Injection mode of multi-mode imaging of urinary system and blood vessels. ICG is injected into the blood vessels and L1057 NPs are injected into the urinary system. c, Multi-mode pseudo color imaging of ureters and blood vessels of the rat treated with ICG and L1057 NPs under the excitation of a 915 nm CW laser. In channel 1, the blood vessels present bright positive visualization (displayed in pseudo color green), and the ureters which absorb the background ASF of ICG present obvious dark negative visualization (displayed in pseudo color pink), achieving automatic two-organ simultaneous visualization. Meanwhile, in channel 2, only ureters have signals (displayed in pseudo color pink), achieving the single-target visualization.
Credit
by Jing Zhou, Xiaoxiao Fan, Di Wu, Jie Liu, Yuhuang Zhang, Zikang Ye, Dingwei Xue, Mubin He, Liang Zhu, Zhe Feng, Andrey N. Kuzmin, Wen Liu, Paras N. Prasad, Jun Qian
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License
CC BY