Inactivation of the Expression of 3 Key Regulators (IMAGE)
Caption
Inactivation of the expression of three key regulators bfiS, bfmR, and mifR in mature biofilms results in biofilm architectural collapse and biomass loss. P. aeruginosa mutants complemented with plasmid-borne copies of the respective genes placed under the regulation of the arabinose-inducible PBAD were grown under continuous flow conditions in glutamate minimal medium in the presence of 0.1% arabinose for 144 hr after which time the biofilms were visualized by confocal microscopy (0 hr). Then, arabinose was eliminated from the growth medium and the biofilm architecture monitored post arabinose removal at the times indicated. PAO1 strain harboring the empty pJN105 vector was used as control. White bars = 100 μm.
Credit
Karin Sauer
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With permission from Professor Sauer.
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