Figure 1| Direct coupling of Stimulated emission depletion (STED) and Stimulated Raman excited fluorescence (SREF). (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure 1| Direct coupling of Stimulated emission depletion (STED) and Stimulated Raman excited fluorescence (SREF).

Caption

a, Energy diagram of STED-SREF. b, Spectroscopy configuration of STED-SREF. The nitrile mode of Rhodamine 800 (Rh800) is used here. c, SREF/STED-SREF imaging of Rh800 stained E. coli cells with pump wavelengths set at 836 nm (off resonance of the nitrile mode) and 838 nm (vibrational resonance of the nitrile mode). Direct coupling of stimulated emission depletion (STED) with SREF imaging fails to achieve desired resolution improvements. The anti-Stokes fluorescence background (shown in SREF pump=836 nm) has an undesired role in preventing direct adoption of super-resolution fluorescence technique.

Credit

by Hanqing Xiong†, Naixin Qian†, Yupeng Miao, Zhilun Zhao, Chen Chen, Wei Min.

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