Figure 2| Background-free SREF imaging through frequency modulation with high sensitivity and sharp vibrational contrast. (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure 2| Background-free SREF imaging through frequency modulation with high sensitivity and sharp vibrational contrast.

Caption

a, the raw SREF spectrum of Rh800 nitrile mode acquired by conventional SREF excitation (red curve) and corresponding background-free FM-SREF spectrum acquired by FM-SREF excitation. b, the FM-SREF imaging and electronic pre-resonance stimulated Raman scattering (epr-SRS) imaging of Rh800 stained E. coli cells. c, the corresponding intensity distribution for FM-SREF and epr-SRS along the corresponding white dashed lines in (b). d, chemical structures of the two SREF dyes employed. e, the absorption spectra (solid curves) and emission spectra (dash curves) of the two dyes in water, which are not resolvable for conventional fluorescence spectroscopy. f, the FM-SREF spectra of the nitrile modes of the two dyes. g, multicolor FM-SREF imaging of S. cerevisiae cells.

Credit

by Hanqing Xiong†, Naixin Qian†, Yupeng Miao, Zhilun Zhao, Chen Chen, Wei Min.

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