14 Ways to Slice a Tomato (Gene Promoter) (IMAGE) Cold Spring Harbor Laboratory Caption This graphic plots changes in gene promoter SlCLV3 induced by making cuts in its DNA sequence with CRISPR gene editing technology. The relative location of 8 guide RNAs used to target cuts in the wild-type (unmutated) sequence of the promoter are represented in the top line by blue arrowheads. After the cuts are made, cellular DNA repair machinery addresses the damage, a process that results in different "repaired" variations of the sequence. Here, 14 different variants, or alleles are listed on the Y axis; moving across each variant sequence from left to right, we see positions where additions and/or deletions of DNA resulted from the CRISPR cuts following repair. The impact of each of these 14 variants of the promoter's sequence upon the number of seed compartments, or locules, in the plant is indicated by the color-coded readout at the far right. They range from 2-3 locules in the wild-type tomato to 7 or more in the m4 and s6 variant alleles. Credit Lippman lab, CSHL Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.