Imaging hiPSC-derived Retinal Organoids with D-FFOCT (IMAGE)
Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS
Caption
a, Colour bar of the D-FFOCT images with a consistent colormap for (b,c). b, Image of a D29 retinal organoid, showing multiple cells with different dynamic profiles. c, Image of a D51 retinal organoid, where precursors of photoreceptors begin to appear in a rosette formation (red dotted line). High-temporal-resolution imaging performed on a D147 retinal organoid. d, Part of the retinal organoid revealed fusiform structures corresponding to emerging photoreceptor outer segments in the centre of the rosette. e, Magnified view of nuclei in three different states around the rosette: (i) a nucleus in a normal state with a compact, uniform shape and is very bright (i.e., exhibiting a high activity); (ii) an seemingly dying, inflated nucleus, exhibiting almost no activity; and (iii) a nucleus undergoing division with no defined nuclear membrane in the cytoplasm, and two distinct parts (white arrows) of the content of a nucleus (suggesting mitosis of the nucleus with chromosomes already divided, with the same subcellular activity level as the "normal" nucleus). f, Magnified image of the photoreceptor outer segment-like structures imaged side-on; three of them are marked with a white line. Scale bar: 20 μm.
Credit
by Jules Scholler, Kassandra Groux, Olivier Goureau, José-Alain Sahel, Mathias Fink, Sacha Reichman, Claude Boccara and Kate Grieve
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