Imaging Modalities of FFOCT (IMAGE)
Caption
a, Setup to create FFOCT and D-FFOCT images, combined with fluorescence for validation. b, 3D representation of a portion of a 28 day old (D28) hiPSC-derived retinal organoid captured with D-FFOCT, with the corresponding colorbar. c, Comparison of FFOCT and D-FFOCT images of a D29 retinal organoid: the FFOCT image shows the global structure of the sample, whereas the D-FFOCT image reveals the different cells constituting the sample, with much higher contrast. Scale-bar: 20 μm.
Credit
by Jules Scholler, Kassandra Groux, Olivier Goureau, José-Alain Sahel, Mathias Fink, Sacha Reichman, Claude Boccara and Kate Grieve
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