Pusan National University scientists explore OsMATL2 gene as a candidate for haploid induction in rice

Conventional crop breeding is not time-efficient for creating inbred lines with desired genetic traits owing to the diploid nature of plants, wherein they have two sets of chromosomes, one from each parent. In contrast, double haploid technology utilizes gene-edited haploid inducer plants to create double haploid crops that have two sets of chromosomes from a single parent. This revolutionary agricultural method can create inbred crop lines in a single generation thereby accelerating the breeding process.

Recent studies have unveiled the potential of specific genes in triggering haploid induction, a key step in double haploid technology. In particular, the gene ZmMATL was identified as a pollen-specific phospholipase in maize that plays a role in haploid induction within the plant's reproductive processes. Building on this discovery, researchers demonstrated the conservation of pollen-specific phospholipase A-mediated in vivo haploid induction across various monocot species including indica rice by mutating the OsMATL gene. However, the haploid induction rate (HIR) was found to be only 6% at best, which is far below the industry standard, suggesting a need to increase this rate.

In a recent collaborative study, researchers from Korea led by Dr. Yu-Jin Kim from Pusan National University identified OsMATL2, a potential haploid-inducing gene in Japonica rice (Oryza sativa japonica). Their research was published online on July 20, 2023, in Plant Physiology.

Using a combination of GUS reporter genes, green fluorescent protein-tagged antibodies, and reverse transcriptase quantitative polymerase chain reaction, the team observed that OsMATL2 protein, a phospholipase enzyme, is highly expressed in pollen, primarily in the plasma membrane of cells of the japonica rice plant.

To evaluate the role of OsMATL2—the gene encoding OsMATL2 protein—in haploid induction, the team used the CRISPR/Cas9 system to generate knockout mutants (sgOsMATL2) with suppressed OsMATL2 protein expression. While diploid sgOsMATL2 plants showed no vegetative defects compared to normal rice, their haploid counterparts were notably smaller in size. Additionally, all sgOsMATL2 plants showed reduced (around 80% of normal) seed setting. Most importantly, haploid sgOsMATL2 plants showed male sterility due to severe defects in pollen development.

Through flow cytometry and fluorescence microscopy, the researchers observed that haploid sgOsMATL2 plants possessed only half as many chromosomes as normal, confirming that inactivation of the OsMATL2 gene does indeed trigger haploid induction. The HIR of sgOsMATL2 plants was observed at 6.34% on average, which is slightly higher than the HIR triggered by OsMATL mutation. “Traditional crop breeding requires multiple generations of self-crossing. Gene-edited OsMATL2 plants can be used as a haploid inducer to produce perfectly inbred rice lines in a single generation”, Dr. Kim summarized.

This study showed the existence of a new haploid-inducing gene in rice. While OsMATL and OsMATL2 genes have an individual HIR of ~6%, with their functional redundancy, it may be possible to mutate both genes to obtain a higher HIR. The applications of this research can revolutionize rice cultivation.      “Rapid crop breeding is required to combat climate change, abiotic stress, and threats from viruses. Identification of more haploid-inducing genes and understanding their mechanisms during double fertilization in plants can reduce the time and effort needed for effective crop breeding”, concludes Dr. Kim

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Reference      

DOI: https://doi.org/10.1093/plphys/kiad422

About the Institute

Pusan National University, located in Busan, South Korea, was founded in 1946 and is now the No. 1 national university of South Korea in research and educational competency. The multi-campus university also has other smaller campuses in Yangsan, Miryang, and Ami. The university prides itself on the principles of truth, freedom, and service, and has approximately 30,000 students, 1200 professors, and 750 faculty members. The university comprises 14 colleges (schools) and one independent division, with 103 departments in all.
Website: https://www.pusan.ac.kr/eng/Main.do

About the author

Yu-Jin Kim is an Assistant Professor at the Department of Life Science and Environmental Biochemistry at Pusan National University. She received her Ph.D. from Kyung Hee University, after which she did her post-doctoral research at Shanghai Jiao Tong University, China. Her lab focuses on molecular research of plant development and response to environments. She intends to contribute to the increase of crop production and develop environmental stress-controlled crops by identifying and functionally characterizing key genes through system biology approaches and genetic engineering techniques such as CRISPR-Cas9. Recently, her group is developing approaches to investigate male and female factors for rice fertilization.

Lab: https://life-pmb.pusan.ac.kr/life-pmb/index..do

ORCID id: 0000-0003-2562-615