News Release

Female lab mice behave very differently when placed outdoors

Peer-Reviewed Publication

Cornell University

ITHACA, N.Y. – A new Cornell University study is the first to examine social behavior of lab mice in large outdoor enclosures.

The research, published in the journal BMC Biology, took lab mice and placed them in large outdoor enclosures. The researchers found that male behavior was essentially the same as genetically wild mice, but females displayed radically different behaviors.

“Given how much of biomedical research is carried out on a single strain of lab mice, it is critical that we understand them as organisms,” said senior author Michael Sheehan, associate professor of neurobiology and behavior.

“The findings have broad implications for all biomedical studies using lab mice but especially those that use mice as a model of social behavior,” Sheehan said. At the same time, the study’s insights don’t necessarily invalidate previous research, he said.

In the study, the researchers compared the most studied lab mouse strain – genetically uniform C57BL/6J – against house mice whose ancestors were caught in upstate New York and have been outbred so every individual is genetically distinct.

In each replicate trial, 10 male and 10 female C57 mice were placed in 5,000-square-foot enclosures for a 10-day trial and were compared to control trials with genetically wild mice.

After collecting 9 million data points, the researchers found that male C57 mice and wild mice behaved similarly to each other when placed outdoors. In the lab, mice are housed with other mice in shoe box-sized cages. When space is limited, they create a hierarchy where a dominant male rules the area. But when placed outdoors, males will establish their own territories.

“Which is exactly what people have reported from wild mouse populations: When you give them space, a fundamental feature of their social structure is that they will make territories – a behavior that we simply can’t study in the lab,” Sheehan said.

Much like males, when wild females have plenty of space, they avoid each other as they compete for food or nesting resources. They differ from males in that sometimes multiple females will share a space together, and when they do, it is with sisters. The lab females in spacious enclosures, on the other hand, often go everywhere, every night.

“The lab females also don’t show any preferential interactions with their sisters, they essentially interact randomly with every other female,” Sheehan said.

Since all the lab mice are genetically identical, it is hard to know whether the behavioral differences between wild and lab mice occur because it is harder for individual lab mice to tell each other apart or if there is a more fundamental difference to their social structures, Sheehan said.

The study also revealed that when left to themselves with plenty of space, the mice choose to spend more than half their time alone. In the lab, mice spend their entire lives in small cages with up to four other mice. The findings could have relevance on studies that examine social isolation as a predictor of health, Sheehan said.

The researchers also found that these results were highly repeatable between trials. Despite all the complexity of social interactions in large groups, given the same initial starting conditions the two genotypes reliably produced different social structures.

“It opens the door to experimentally manipulate the demographic or genetic composition of free-living populations to ask questions about how realistic natural social environments affect health, fitness and life outcomes for individuals,” Sheehan said.

The study was funded by the U.S. Department of Agriculture and the National Institutes of Health.

For additional information, see this Cornell Chronicle story.

Cornell University has dedicated television and audio studios available for media interviews.


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