News Release

Blended antioxidant supplement improves cognition and memory in aged mice

Scientists find that mice administered with blended antioxidant supplement showed significant improvements in spatial cognition, short-term memory, and muscle durability

Peer-Reviewed Publication

Shibaura Institute of Technology

Blended antioxidant supplement improves spatial cognition in mice


Administration of Twendee X (TwX) improves spatial learning ability in aged mice. The average goal time (escape latency) in the spatial cognition test is shown in (A). The percentage of time spent in the quadrant is shown in (B). The average swimming speed on Day 5 of the experiment is shown in (C).

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Credit: Koji Fukui from SIT, Japan

Age-related decline in cognitive and muscle function continues to be a significant challenge for the field of healthcare. Healthcare costs associated with treating age-related cognitive decline and muscle weakness are expected to increase substantially in the future. One of the primary underlying mechanisms responsible for age-related health decline is oxidative stress, which refers to the progressive damage inflicted by oxygen-free radicals on cells.

Certain compounds in foods, known as antioxidants, are capable of neutralizing oxygen-free radicals. Consuming antioxidant-rich foods is known to reduce cell damage and slow down age-related health decline. In the absence of an antioxidant-rich diet, people often turn to antioxidant supplements that offer comparable or greater health protection. Now, a team of scientists, led by Professor Koji Fukui affiliated with the Shibaura Institute of Technology (SIT) and including Dr. Fukka You from Gifu University found that administering a blended mix of antioxidant supplements to aged mice significantly improves their spatial cognition, short-term memory, and muscle durability. The paper was published in the special Issue ‘Antioxidants in health and diseases’ of the International Journal of Molecular Sciences on February 28, 2024.

In this study, significant improvements were observed in the spatial learning ability and short-term memory in supplement-treated aged mice. Long term intake of blended antioxidant supplements may be effective, even considering the effects of aging and related increased oxidation in the body,”, explains Prof. Fukui, the lead researcher of the study. Memory loss is associated with several debilitating diseases, such as Alzheimer’s, which disproportionately affect older people. The discovery that blended antioxidant supplements improve memory in mice suggests that they may also be beneficial in preventing memory loss in humans.

Sarcopenia, another age-related disease, results in a progressive loss of muscle strength in older individuals. This condition significantly affects people’s mobility, often leading to social isolation. Moreover, sarcopenia can increase the risk of developing cognitive disorders. If blended antioxidant supplements can enhance muscle strength in mice, they may also hold the potential for mitigating muscle frailty and sarcopenia in humans.

Frailty and sarcopenia are now serious problems and potent risk factors for dementia. Although the mechanism is unknown, it is groundbreaking that taking supplements may be able to prevent muscle weakness”, notes Prof. Fukui.

Numerous types of antioxidant supplements are available in the market, and determining the right supplements to buy can often be challenging for consumers. The results of this groundbreaking study by Professor Fukui and his colleagues support the use of blended antioxidant supplements to prevent age-related health decline. However, further research is necessary to establish the efficacy and safety of blended antioxidant supplements in humans. Moreover, specific antioxidant blends may have varying effects on the human body, and their use should be ideally based on clinical evidence. The antioxidant blend used in the study was Twendee X, which has a similar composition to the commercially available supplement Oxycut®.

Although many types of antioxidant supplements are available, the effect is greater if multiple types are taken simultaneously rather than one type. However, it is difficult to know which type and how much to take, as it is possible to take too many of some vitamins,” Prof. Fukui observes. “We recommend only taking multivitamins that are guaranteed to be safe,” he cautions.

Besides choosing the right antioxidant supplement, adopting the right regimen can also confuse consumers. Future research on the individual differences in the effects of antioxidants can reduce confusion around the optimum dose and composition of antioxidant supplements. Over the long term, optimal use of antioxidant supplements may significantly reduce age-related health decline. “In the future, there will come a time when we will provide multi-supplements tailored to each individual. There will be no need to worry about overdosing,” concludes Prof. Fukui.





Title of original paper: A Blended Vitamin Supplement Improves Spatial Cognitive and Short-Term Memory in Aged Mice

Journal: International Journal of Molecular Sciences



About Shibaura Institute of Technology (SIT), Japan

Shibaura Institute of Technology (SIT) is a private university with campuses in Tokyo and Saitama. Since the establishment of its predecessor, Tokyo Higher School of Industry and Commerce, in 1927, it has maintained “learning through practice” as its philosophy in the education of engineers. SIT was the only private science and engineering university selected for the Top Global University Project sponsored by the Ministry of Education, Culture, Sports, Science and Technology and will receive support from the ministry for 10 years starting from the 2014 academic year. Its motto, “Nurturing engineers who learn from society and contribute to society,” reflects its mission of fostering scientists and engineers who can contribute to the sustainable growth of the world by exposing their over 8,000 students to culturally diverse environments, where they learn to cope, collaborate, and relate with fellow students from around the world.



About Professor Koji Fukui from SIT, Japan

Dr. Koji Fukui is currently a Professor at Shibaura Institute of Technology, Japan. He has 24 years of research experience and has published over 87 scientific articles. Professor Fukui’s research mainly explores the relationship between oxidative damage and brain aging, as well as, the protective effects of antioxidants in the brain and body. He obtained his Ph.D. from Shibaura Institute of Technology in 2003 and has since worked at various research institutes such as Wakayama Medical University and Hokkaido University.


Funding Information

This research was conducted under a joint research agreement between Shibaura Institute of Technology and Gifu University. The research funding was provided by the Division of Anti-oxidant Research, Life Science Research Center, Gifu University.

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