Hornets in town: How top predators coexist

In urban environments, competing hornet species coexist by specializing on different prey species. The Kobe University study was made possible by pioneering DNA analysis of hornet larvae's gut contents and shows that cities are fascinating model systems for how predatory species adapt to environmental stress.

City gangs brutally illustrate a principle that is a staple in ecological theory, the “competitive exclusion principle”: Two species competing for the same limited resource cannot coexist. Nature seems to find more peaceful solutions. “The yellow-vented hornet and the Japanese yellow hornet are both considered urban adapters with nesting sites and activity periods largely overlapping. Top predators like these should not coexist. And yet, they both thrive in urban areas,” says Kobe University entomologist SAGA Tatsuya. The details of how they achieve this, however, remained shrouded in mystery, probably largely because visual observation of hornet feeding habits is difficult and insufficient.

Saga recounts an event that led him to coming up with a solution, saying: “While working in a region where people observed the custom of eating giant hornet larvae, I had the opportunity to eat one myself. I cannot forget the unique, gritty texture of the intestinal contents. At that moment, it occurred to me that extracting the DNA from the larvae’s intestinal contents might finally reveal what hornets eat.” And so, he developed a technique that applies “DNA metabarcoding,” sampling DNA from a specific environment and categorizing it into taxonomic groups, to larva contents acquired from hornet nests that were collected during exterminations across Kobe and its environs.

In the journal Entomologia Generalis, Saga and his collaborator (now at Kyushu University) now publish what they found: The two hornet species divided their prey by specializing on different targets. While the Japanese yellow hornet ate soft-bodied prey like crickets and moths, the yellow-vented hornet mostly consumed “low-value prey” such as beetles and other wasps. Saga says, “We were surprised to see this pattern, because the yellow-vented hornet usually displaces the Japanese yellow hornet in sap-feeding experiments and is thus considered more competitive.”

All this is even more interesting since the entomologists conducted their analysis both in urban and non-urban environments and found that away from town, the two species actually consumed similar prey. However, going from non-urban to urban environments, it was the “more dominant” yellow-vented hornet that changed its food habits more drastically than the Japanese yellow hornet. This means that resource dominance is possibly less suited to predicting a predator’s success under stress than its ability to adapt its prey choice. “These changes show that cities act as trait filters that drive niche differentiation by selecting on traits that confer resilience under stress,” explains Saga.

Urbanization is not only a rapidly advancing, global process, it is also a process that occurs consistently across a broad range of climates and regions. These processes include habitat simplification and resource fragmentation that make urban habitats “valuable model systems for the understanding of broader patterns of environmental change,” as the researchers write. In addition, Saga hopes his study may lead to a new understanding of hornets, saying: “The study provides the data to see hornets not merely as dangerous pests but as important ecosystem regulators.”

This research was funded by the Coop Kobe Environmental Fund, the Hokuto Foundation for Bioscience, the Sumitomo Foundation Environmental Research Projects (grant 2432129) and the Environmental Restoration and Conservation Agency Japan Society for the Promotion of Science (grant JPMEERF20254RB1). It was conducted in collaboration with a researcher from Kyushu University.

Kobe University is a national university with roots dating back to the Kobe Higher Commercial School founded in 1902. It is now one of Japan’s leading comprehensive research universities with over 16,000 students and over 1,700 faculty in 11 faculties and schools and 15 graduate schools. Combining the social and natural sciences to cultivate leaders with an interdisciplinary perspective, Kobe University creates knowledge and fosters innovation to address society’s challenges.