News Release

ClC-3 chloride channel in hippocampal neuronal apoptosis

Peer-Reviewed Publication

Neural Regeneration Research

CIC-3 Positive Neurons

image: CIC-3 positive neurons in the SIN-1 group were significantly increased in a hippocampal neuronal apoptotic model. view more 

Credit: <i>Neural Regeneration Research</i>

Over-production of nitric oxide and ion disturbance are involved in neuronal apoptosis around the ischemic area following ischemic brain injury. Prof. Quanzhong Chang and team from Zhuhai Campus of Zunyi Medical College in China established a rat model of hippocampal neuronal apoptosis by using 3-morpholinosyndnomine (SIN-1), a nitric oxide donor. The models were then administered the chloride channel blocker, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), in a broader attempt to observe ClC-3 chloride channel expression in the neuronal apoptosis process and to investigate the correlation between chloride channel activity and ischemia-sensitive neuronal apoptosis. Their results showed that SIN-1 reduced the neuronal survival rate, induced neuronal apoptosis, and promoted ClC-3 chloride channel protein and mRNA expression in the apoptotic neurons. DIDS reversed the effect of SIN-1. These findings, published in the Neural Regeneration Research (Vol. 8, No. 32, 2013), indicate that the increased activities of the ClC-3 chloride channel may be involved in hippocampal neuronal apoptosis induced by nitric oxide.


Article: " ClC-3 chloride channel in hippocampal neuronal apoptosis " by Lijuan Xu1, 2, Shuling Zhang3, Hongling Fan1, Zhichao Zhong1, Xi Li1, Xiaoxiao Jin1, Quanzhong Chang 1 (1 Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China; 2 First Hospital of Putian City, Putian 351100, Fujian Province, China; 3 Department of Pediatrics, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China)

Xu LJ, Zhang SL, Fan HL, Zhong ZC, Li X, Jin XX, Chang QZ. ClC-3 chloride channel in hippocampal neuronal apoptosis. Neural Regen Res. 2013;8(32):3047-3054.


Meng Zhao
Neural Regeneration Research

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