WASHINGTON, Aug. 22, 2017 -- Imagine you're on your way to Mars, and you lose a crucial tool during a spacewalk. Not to worry, you'll simply re-enter your spacecraft and use some microorganisms to convert your urine and exhaled carbon dioxide (CO2) into chemicals to make a new one. That's one of the ultimate goals of scientists who are developing ways to make long space trips feasible.
The researchers are presenting their results today at the 254th National Meeting & Exposition of the American Chemical Society (ACS). ACS, the world's largest scientific society, is holding the meeting here through Thursday. It features nearly 9,400 presentations on a wide range of science topics.
A brand-new video on the research is available at http://bit.ly/acsmars.
Astronauts can't take a lot of spare parts into space because every extra ounce adds to the cost of fuel needed to escape Earth's gravity. "If astronauts are going to make journeys that span several years, we'll need to find a way to reuse and recycle everything they bring with them," Mark A. Blenner, Ph.D., says. "Atom economy will become really important."
The solution lies in part with the astronauts themselves, who will constantly generate waste from breathing, eating and using materials. Unlike their friends on Earth, Blenner says, these spacefarers won't want to throw any waste molecules away. So he and his team are studying how to repurpose these molecules and convert them into products the astronauts need, such as polyesters and nutrients.
Some essential nutrients, such as omega-3 fatty acids, have a shelf life of just a couple of years, says Blenner, who is at Clemson University. They'll need to be made en route, beginning a few years after launch, or at the destination. "Having a biological system that astronauts can awaken from a dormant state to start producing what they need, when they need it, is the motivation for our project," he says.
Blenner's biological system includes a variety of strains of the yeast Yarrowia lipolytica. These organisms require both nitrogen and carbon to grow. Blenner's team discovered that the yeast can obtain their nitrogen from urea in untreated urine. Meanwhile, the yeast obtain their carbon from CO2, which could come from astronauts' exhaled breath, or from the Martian atmosphere. But to use CO2, the yeast require a middleman to "fix" the carbon into a form they can ingest. For this purpose, the yeast rely on photosynthetic cyanobacteria or algae provided by the researchers.
One of the yeast strains produces omega-3 fatty acids, which contribute to heart, eye and brain health. Another strain has been engineered to churn out monomers and link them to make polyester polymers. Those polymers could then be used in a 3-D printer to generate new plastic parts. Blenner's team is continuing to engineer this yeast strain to produce a variety of monomers that can be polymerized into different types of polyesters with a range of properties.
For now, the engineered yeast strains can produce only small amounts of polyesters or nutrients, but the scientists are working on boosting output. They're also looking into applications here on Earth, in fish farming and human nutrition. For example, fish raised via aquaculture need to be given omega-3 fatty acid supplements, which could be produced by Blenner's yeast strains.
Although other research groups are also putting yeast to work, they aren't taking the same approach. For example, a team from DuPont is already using yeast to make omega-3 fatty acids for aquaculture, but its yeast feed on refined sugar instead of waste products, Blenner says. Meanwhile, two other teams are engineering yeast to make polyesters. However, unlike Blenner's group, they aren't engineering the organisms to optimize the type of polyester produced, he says.
Whatever their approach, these researchers are all adding to the body of knowledge about Y. lipolytica, which has been studied much less than, say, the yeast used in beer production. "We're learning that Y. lipolytica is quite a bit different than other yeast in their genetics and biochemical nature," Blenner says. "Every new organism has some amount of quirkiness that you have to focus on and understand better."
A press conference on this topic will be held Tuesday, Aug. 22, at 2 p.m. Eastern time in the Walter E. Washington Convention Center. Reporters may check-in at the press center, Room 154A, or watch live on YouTube http://bit.ly/ACSLive_DC. To ask questions online, sign in with a Google account.
Blenner's work was supported by an Early Career Faculty grant from NASA's Space Technology Research Grants Program.
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Biosynthesis of materials and nutraceuticals from astronaut waste: Towards closing the loop
The journey to Mars will be longer than any mission before. Astronaut needs for materials, nutraceuticals, and life support systems will critical. However, future missions may benefit from flexible, programmable, low power, and sustainable manufacturing processes driven by resources available during the mission. These resources include in situ minerals and feedstocks, solar energy, and waste generated by crew and space shuttle operation. Microbiological systems are well suited for utilizing such ill-defined resources and reliably manufacturing both specific and complex molecules. Our work is focused on using synthetic biology and metabolic engineering to develop Y. lipolytica as a platform organism to produce omega-3 fatty acids and polyhydroxyalkonoates (PHAs) from autotrophic cell waste and human urine. Our work demonstrates that Y. lipolytica is not only capable of using urine as a nitrogen source but it also grows more efficiently and produces higher lipid concentrations. Benefits and challenges of using waste substrates will be discussed and compared to defined media. Together, our work moves us closer to biomanufacturing for astronaut needs.