SALT LAKE CITY - Why elephants rarely get cancer is a mystery that has stumped scientists for decades. A study led by researchers at Huntsman Cancer Institute (HCI) at the University of Utah and Arizona State University, and including researchers from the Ringling Bros. Center for Elephant Conservation, may have found the answer.
According to the results, published today in the Journal of the American Medical Association (JAMA), and determined over the course of several years and a unique collaboration between HCI, Primary Children's Hospital, Utah's Hogle Zoo, and the Ringling Bros. Center for Elephant Conservation, elephants have 38 additional modified copies (alleles) of a gene that encodes p53, a well-defined tumor suppressor, as compared to humans, who have only two. Further, elephants may have a more robust mechanism for killing damaged cells that are at risk for becoming cancerous. In isolated elephant cells, this activity is doubled compared to healthy human cells, and five times that of cells from patients with Li-Fraumeni Syndrome, who have only one working copy of p53 and more than a 90 percent lifetime cancer risk in children and adults. The results suggest extra p53 could explain elephants' enhanced resistance to cancer.
"Nature has already figured out how to prevent cancer. It's up to us to learn how different animals tackle the problem so we can adapt those strategies to prevent cancer in people," says co-senior author Joshua Schiffman, M.D., pediatric oncologist at Huntsman Cancer Institute, University of Utah School of Medicine, and Primary Children's Hospital.
According to Schiffman, elephants have long been considered a walking conundrum. Because they have 100 times as many cells as people, they should be 100 times more likely to have a cell slip into a cancerous state and trigger the disease over their long life span of 50 to 70 years. And yet it's believed that elephants get cancer less often, a theory confirmed in this study. Analysis of a large database of elephant deaths estimates a cancer mortality rate of less than 5 percent compared to 11 to 25 percent in people.
In search of an explanation, the scientists combed through the African elephant genome and found at least 40 copies of genes that code for p53, a protein well known for its cancer-inhibiting properties. DNA analysis provides clues as to why elephants have so many copies, a substantial increase over the two found in humans. The vast majority, 38 of them, are so-called retrogenes, modified duplicates that have been churned out over evolutionary time.
Schiffman's team collaborated with Utah's Hogle Zoo and Ringling Bros. Center for Elephant Conservation to test whether the extra gene copies may protect elephants from cancer. They extracted white blood cells from blood drawn from the animals during routine wellness checks and subjected the cells to treatments that damage DNA, a cancer trigger. In response, the cells reacted to damage with a characteristic p53-mediated response: they committed suicide.
"It's as if the elephants said, 'It's so important that we don't get cancer, we're going to kill this cell and start over fresh,'" says Schiffman. "If you kill the damaged cell, it's gone, and it can't turn into cancer. This may be more effective of an approach to cancer prevention than trying to stop a mutated cell from dividing and not being able to completely repair itself."
With respect to cancer, patients with inherited Li-Fraumeni Syndrome are nearly the opposite of elephants. They have just one active copy of p53 and more than a 90 percent lifetime risk for cancer. Less p53 decreases the DNA damage response in patients with Li-Fraumeni Syndrome, and Schiffman's team wondered if more p53 could protect against cancer in elephants by heightening the response to damage. To test this, the researchers did a side-by-side comparison with cells isolated from elephants (n=8), healthy humans (n=10), and from patients with Li-Fraumeni Syndrome (n=10). They found that elephant cells exposed to radiation self-destruct at twice the rate of healthy human cells and more than five times the rate of Li-Fraumeni cells (14.6%, 7.2%, and 2.7%, respectively). These findings support the idea that more p53 offers additional protection against cancer.
"By all logical reasoning, elephants should be developing a tremendous amount of cancer, and in fact, should be extinct by now due to such a high risk for cancer," says Schiffman. "We think that making more p53 is nature's way of keeping this species alive." Additional studies will be needed to determine whether p53 directly protects elephants from cancer.
"Twenty years ago, we founded the Ringling Bros. Center for Elephant Conservation to preserve the endangered Asian elephant for future generations. Little did we know then that they may hold the key to cancer treatment," said Kenneth Feld, Chairman and CEO of Feld Entertainment.
"The incredible bond our staff has with these majestic animals, and the hands-on care provided at the Center for Elephant Conservation, allows us to easily provide the blood samples Dr. Schiffman needs to further his research," said Alana Feld, executive vice president of Feld Entertainment and producer of Ringling Bros. and Barnum & Bailey. "We look forward to the day when there is a world with more elephants and less cancer."
The elephant story represents one way that evolution may have overcome cancer. Other evidence suggests that naked mole rats and bowhead whales have evolved different approaches to the problem. Schiffman plans to use what he's learned in elephants as a strategy for developing novel cancer-fighting therapies.
Schiffman and co-authors, Lisa Abegglen, Ashley Chan, Kristy Lee, Rosann Robinson, Michael Campbell, and Srividya Bhaskara are from Huntsman Cancer Institute and the University of Utah, Aleah Caulin and Shane Jensen are from the University of Pennsylvania, Wendy Kiso and Dennis Schmitt are from the Ringling Bros. Center for Elephant Conservation, Peter Waddell is from the Ronin Institute in West Lafayette, Indiana, and Carlo Maley, senior co-author, is from Arizona State University. Also contributing to the research was Eric Peterson, elephant manager at Utah's Hogle Zoo.
"Participating in the research is not only amazing but a win-win for humans and elephants," said Peterson. "If elephants can hold the key to unlocking some of the mysteries of cancer, then we will see an increased awareness of the plight of elephants worldwide. What a fantastic benefit: elephants and humans living longer, better lives."
"The animal kingdom undoubtedly holds information that could help lead to cures for many human illnesses," said Craig Dinsmore, executive director, Utah's Hogle Zoo. "The blood samples from our elephants at Utah's Hogle Zoo are aiding Dr. Schiffman in his research, and we are proud to be a part of his ground-breaking work."
"Potential mechanisms for cancer resistance in elephants and comparative cellular response to DNA damage in humans" will be published in JAMA online on October 8, 2015. For a copy of the paper, email the JAMA Network media relations department at firstname.lastname@example.org.
The work was supported by Huntsman Cancer Institute, the National Cancer Institute, Huntsman Cancer Foundation, Intermountain Healthcare Foundation, Primary Children's Hospital Foundation, Soccer For Hope, Utah's Hogle Zoo, Ringling Bros. and Barnum & Bailey Circus, U.S. Department of Energy, and the Department of Defense.
Huntsman Cancer Institute (HCI) is one of the world's top academic research and cancer treatment centers. HCI manages the Utah Population Database - the largest genetic database in the world, with more than 16 million records linked to genealogies, health records, and vital statistics. Using this data, HCI researchers have identified cancer-causing genes, including the genes responsible for melanoma, colon and breast cancer, and paraganglioma. HCI is a member of the National Comprehensive Cancer Network (a 26-member alliance of the world's leading cancer centers) and is a National Cancer Institute-Designated Comprehensive Cancer Center. HCI treats patients with all forms of cancer and operates several high-risk clinics that focus on melanoma and breast, colon, and pancreas cancers. The HCI Cancer Learning Center for patient and public education contains one of the nation's largest collections of cancer-related publications. The institute is named after Jon M. Huntsman, a Utah philanthropist, industrialist, and cancer survivor.
About Feld Entertainment
Feld Entertainment is the worldwide leader in producing and presenting live touring family entertainment experiences that lift the human spirit and create indelible memories, with 30 million people in attendance at its shows each year. Feld Entertainment's productions have appeared in more than 75 countries and on six continents to date and include Ringling Bros. and Barnum & Bailey®, Monster Jam®, Monster Energy Supercross, AMSOIL Arenacross, Disney On Ice Presented by StonyfieldYoKids Organic Yogurt, Disney Live! Presented by StonyfieldYoKids Organic Yogurt and Marvel Universe LIVE! More information about Feld Entertainment is available online at http://www.feldentertainment.com. Additional information on the Ringling Bros. Center for Elephant Conservation is available online at http://www.ringlingelephantcenter.com.
About Utah's Hogle Zoo
Utah's Hogle Zoo is one of only 228 accredited by the Association of Zoos & Aquariums (AZA). Look for the AZA logo whenever you visit a zoo or aquarium as your assurance that you are supporting a facility dedicated to providing excellent care for animals, a great experience for you, and a better future for all living things. AZA is a leader in global wildlife conservation, and your link to helping animals in their native habitats. For more information visit http://www.aza.org.
Linda Aagard, Huntsman Cancer Institute
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Stephen Payne, Feld Entertainment
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Erica Hansen, Utah's Hogle Zoo
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