Biomedical researchers suspect graphene, a novel nanomaterial made of sheets of single carbon atoms, would be useful in a variety of applications. But no one had studied the interaction between graphene and DNA, the building block of all living things. To learn more, PNNL's Zhiwen Tang, Yuehe Lin and colleagues from both PNNL and Princeton University built nanostructures of graphene and DNA. They attached a fluorescent molecule to the DNA to track the interaction. Tests showed that the fluorescence dimmed significantly when single-stranded DNA rested on graphene, but that double-stranded DNA only darkened slightly – an indication that single-stranded DNA had a stronger interaction with graphene than its double-stranded cousin. The researchers then examined whether they could take advantage of the difference in fluorescence and binding. When they added complementary DNA to single-stranded DNA-graphene structures, they found the fluorescence glowed anew. This suggested the two DNAs intertwined and left the graphene surface as a new molecule.
DNA's ability to turns its fluorescent light switch on and off when near graphene could be used to create a biosensor, the researchers propose. Possible applications for a DNA-graphene biosensor include diagnosing diseases like cancer, detecting toxins in tainted food and detecting pathogens from biological weapons. Other tests also revealed that single-stranded DNA attached to graphene was less prone to being broken down by enzymes, which makes graphene-DNA structures especially stable. This could lead to drug delivery for gene therapy. Tang will discuss this research and some of its possible applications in medicine, food safety and biodefense.
Reference: Zhiwen Tang, Biofunctionalization of Graphene for Biosensing and Imaging, Tuesday, September 22, 3:30 – 5:30 p.m. in Ross Island/Morrison at the Doubletree Lloyd Center, Portland, Ore.
TIPSHEET: Scientists from the Department of Energy's Pacific Northwest National Laboratory will be presenting their research at the 2009 Micro Nano Breakthrough Conference, which runs Sept. 21-23 in Portland, Ore. http://oregonstate.edu/conferences/MNBC/.
This research was funded by PNNL as part of its Transformational Materials Science Initiative, http://materials.pnl.gov/.
Pacific Northwest National Laboratory is a Department of Energy Office of Science national laboratory where interdisciplinary teams advance science and technology and deliver solutions to America's most intractable problems in energy, national security and the environment. PNNL employs 4,200 staff and has an $850 million annual budget. Ohio-based Battelle has managed PNNL since the lab's inception in 1965.