A pair of chemicals used by wallflowers and their kin to ward off predators have evolved to complement each other, with one targeting generalist herbivores and the other targeting specialised herbivores that have become resistant to the generalist defence.
Plants are engaged in an ongoing arms race with the creatures that eat them. They evolve defences to deter plant eaters, while their herbivores evolve counter-defences. The new study, published today in eLife, reveals details of the evolutionary chemical arms race in the wallflower genus Erysimum, a group of flowering plants in the mustard family Brassicaceae.
The first line of defence in all Brassicaceae plants are chemicals called glucosinolates, which are activated when predatory creatures nibble on the plants. More recently, wallflowers have developed a second line of defence by producing chemicals called cardenolides to deter plant-eating creatures that evolved defences to glucosinolates.
"Studying how these two defences evolved in this large genus can help scientists understand the trade-offs that the plants face as they try to defend themselves against multiple enemies," explains lead author Tobias Züst, PhD, Research Associate at the Institute of Plant Sciences, University of Bern, Switzerland.
To do this, Züst and his team sequenced the genome of the wormseed wallflower, a short-lived wild mustard. They next created a detailed family tree for this plant and 47 other wallflower species and compared the diversity and abundance of glucosinolates and cardenolides across these species. They found that the two defences varied independently of each other, and that closely related, geographically co-occurring species shared similar cardenolide traits, but not glucosinolate traits. This is likely a result of separate selective pressures acting on each defence.
"Even though most species co-expressed two different types of potentially costly chemical defences, there was no evidence of a trade-off between glucosinolates and cardenolides," Züst says. "Instead, these two types of chemicals appear to complement each other and do not serve redundant functions."
Züst adds that the emergence of cardenolides corresponds with an explosion in the number of wallflower species, which suggests this second complementary defence may have allowed these plants to succeed and diversify into new habitats.
"Further analysis of the wormseed wallflower genome will be needed to help scientists identify glucosinolate and cardenolide-producing genes in this species, as well as aid our understanding of the function of these chemicals in the evolution of Brassicaceae defences," concludes senior author Georg Jander, Professor at the Boyce Thompson Institute in Ithaca, New York, US.
The paper 'Independent evolution of ancestral and novel defenses in a genus of toxic plants (Erysimum, Brassicaceae)' can be freely accessed online at https://doi.org/10.7554/eLife.51712. Contents, including text, figures and data, are free to reuse under a CC BY 4.0 license.
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Full list of funders and grant numbers for this research:
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (PZ00P3-161472), National Science Foundation (1811965), National Science Foundation (1645256), Triad Foundation, Deutsche Forschungsgemeinschaft (DFG-PE 2059/3-1), Agencia Estata de Investigacion, Spain (CGL2017-86626-C2-2-P), LOEWE Program Insect Biotechnology and Bioresources, Junta de Andalucia Programa Operativo (FEDER 2014-2020 A-RNM-505-UGR18)