Safer CRISPR gene editing with fewer off-target hits

The CRISPR system is a powerful tool for the targeted editing of genomes, with significant therapeutic potential, but runs the risk of inappropriately editing "off-target" sites. However, a new study publishing July 9, 2020 in the open-access journal PLOS Biology by Feng Gu of Wenzhou Medical University, China, and colleagues, shows that mutating the enzyme at the heart of the CRISPR gene editing system can improve its fidelity. The results may provide a therapeutically safer strategy for gene editing than using the unmodified enzyme system.

The CRISPR system employs an enzyme called Cas9 to cleave DNA. Cas9 will cut almost any DNA sequence. Its specificity comes from its interaction with a "guide RNA" (gRNA) whose sequence allows it to bind with the target DNA through base-pair matching. Once it does, the enzyme is activated and the DNA is cut.

The CRISPR system is found in multiple bacterial species; among those commonly used in research, that from Staphylococcus aureus has the advantage of size--unlike some others, its gene is small enough to fit inside a versatile and harmless gene therapy vector called adeno-associated virus, making it attractive for therapeutic purposes.

A key limitation of any of the CRISPR systems, including that from S. aureus, is off-target cleavage of DNA. A guide RNA may bind weakly to a site whose sequence is a close but imperfect match; depending on how close the match is and how tightly the enzyme interacts with the paired gRNA-DNA complex, the enzyme may become activated and cut the DNA wrongly, with potentially harmful consequences.

To explore whether the S. aureus Cas9 could be modified to cleave with higher fidelity to the intended target, the authors generated a range of novel Cas9 mutants and tested their ability to discriminate against imperfect matches while retaining high activity at the intended site. They found one such mutant, which distinguished and rejected single base-pair mismatches between gRNA and DNA, regardless of the target, increasing the fidelity up to 93-fold over the original enzyme. They showed that the mutation affected part of the recognition domain, the region of the enzyme that coordinates contacts between the enzyme and the gRNA-DNA complex. The mutation had the likely effect of weakening those contacts, thus ensuring that only the strongest pairing--which would come from a perfect sequence match--would trigger enzyme activity.

"Avoidance of off-target cleavage is a crucial challenge for development of CRISPR for medical interventions, such as correcting genetic diseases or targeting cancer cells," Gu said. "Our results point the way to developing potentially safer gene therapy strategies."

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In your coverage please use these URLs to provide access to the freely available articles in PLOS Biology: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000747

Citation: Xie H, Ge X, Yang F, Wang B, Li S, Duan J, et al. (2020) High-fidelity SaCas9 identified by directional screening in human cells. PLoS Biol 18(7): e3000747. https://doi.org/10.1371/journal.pbio.3000747

Funding: This work was supported by grants from the Natural Science Foundation of China (81201181 to FG, 81700885 to XG, and 81670882 to ZS, http://www.nsfc.gov.cn/), Project of State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou Medical University (No. J01-20190201 to FG), the Zhejiang Provincial & Ministry of Health research fund for medical sciences (WKJ-ZJ-1828 to JZ), and the Science Technology Project of Zhejiang Province (2017C37176 to FG). This work is supported in part by the Intramural Research Program of the NIH/National Institute of Diabetes and Digestive and Kidney Diseases (https://www.niddk.nih.gov/) (ZIADK075136 to JWZ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

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