Researchers are calling for a randomised clinical trial to investigate the potential role of vitamin D supplementation in improving live birth rates following assisted reproduction treatment (ART).
This follows a review and meta-analysis published today in Human Reproduction, which shows a strong link between low vitamin D concentrations in women and lower live birth rates after ART compared to women who have the right amount of vitamin D in their bodies.
The research was led by Dr Justin Chu, an Academic Clinical Lecturer and Specialist Registrar in Obstetrics and Gynaecology for Tommy's National Centre for Miscarriage Research at the University of Birmingham and Birmingham Women's and Children's NHS Foundation Trust.
Dr Chu, from the University of Birmingham's Institute of Metabolism and Systems Research, cautioned that the findings do not mean that vitamin D supplementation necessarily improves women's chances of having a baby following ART as the research can only show an association.
Dr Chu said: "Although an association has been identified, the beneficial effect of correction of vitamin D deficiency or insufficiency needs to be tested by performing a clinical trial.
"In the meantime, women who want to achieve a successful pregnancy should not rush off to their local pharmacy to buy vitamin D supplements until we know more about its effects. It is possible to overdose on vitamin D and this can lead to too much calcium building up in the body, which can weaken bones and damage the heart and kidneys."
The researchers analysed data from 11 published studies that included 2,700 women who were undergoing ART (in vitro fertilisation (IVF), intracytoplasmic sperm injection (ICSI) and/or frozen embryo transfer) and whose vitamin D status had been checked through blood tests.
They found that live births were a third more likely to occur in women who had the right amount of vitamin D when compared to women who did not. Vitamin D concentrations of more than 75 nanomoles per litre of blood (nmol/L) were considered sufficient, concentrations of less than 75 nmol/L were considered insufficient and less than 50 nmol/L were considered deficient.
A similar result was seen when the researchers looked at the results of pregnancy tests and clinical pregnancies (where a foetal heart beat could be detected). When compared with women who had deficient or insufficient concentrations of vitamin D, women who had sufficient vitamin D were 34% more likely to have a positive pregnancy test and 46% more likely to achieve a clinical pregnancy. No associations were found between miscarriage and vitamin D concentrations.
Co-author, Dr Ioannis Gallos, Academic Clinical Lecturer and Subspecialist Trainee in Reproductive Medicine and Surgery at the same institutions, said: "One startling finding was the high prevalence of vitamin D deficiency among these women. We found that only 26% of women in the studies had sufficient concentrations of vitamin D; 35% had deficient concentrations and 45% had insufficient concentrations."
The main source of vitamin D for people is sunlight. In the winter months it can be hard to get enough vitamin D and, in addition, some people are more at risk of vitamin D deficiency, for example those who don't get outdoors much, people with dark skin from African, African-Caribbean and south Asian backgrounds, and people who wear clothes that cover most of their skin when they are outdoors. Other studies have shown that there are higher conception rates in summer and autumn when women might be expected to have more vitamin D due to exposure to summer sunshine. Foods such as oily fish, red meat, liver and egg yolks also provide vitamin D, as well as vitamin D supplements.
The researchers say that possible mechanisms for the role played by vitamin D in pregnancy may be that it affects the success of embryo implantation in the womb in some way or that it is indication of the general well-being of the women.
"Testing for vitamin D concentrations is relatively cheap and widely available and its treatment is not costly," said Dr Chu. "It could be that correcting vitamin D deficiency could benefit women undergoing assisted reproduction treatment, but further research is needed to test this."
The study had some limitations. These included the fact that the studies analysed were often quite different and that vitamin D was assessed before ART was started in some studies and at the time that eggs were retrieved from the women in other studies.
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NOTES TO EDITORS
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- Chu et al (2017). 'Vitamin D and assisted reproductive treatment outcome: a systematic review and meta-analysis'. Human Reproduction. doi:10.1093/humrep/dex326.
- Embargoed pdf of the full paper before the publication date can be obtained at: https://academic.oup.com/DocumentLibrary/humrep/PR_Papers/dex326.pdf
- N.B. Journalists: please don't use this link in any stories that you publish. It is for the exclusive use of the media BEFORE publication. After publication the paper will be replaced, so please link to the published paper on the Human Reproduction website at: https://academic.oup.com/humrep/advance-articles
- Human Reproduction is a monthly journal of the European Society of Human Reproduction and Embryology (ESHRE), and is one of the top three journals in the world in the field of reproductive biology, obstetrics and gynaecology. It is published by Oxford Journals, a division of Oxford University Press. Please acknowledge Human Reproduction as a source in any articles. ESHRE's website is: http://www.eshre.eu
- Abstracts of other papers in ESHRE's three journals - Human Reproduction, Molecular Human Reproduction and Human Reproduction Update - can be accessed post embargo from: https://academic.oup.com/humrep, https://academic.oup.com/molehr, https://academic.oup.com/humupd
- The authors would like to acknowledge Derick Yates (Birmingham Women's and Children's NHS Foundation Trust) who helped design the search strategy for the systematic review and meta-analysis.
- The research was carried out by individuals affiliated to:
1. Tommy's National Centre for Miscarriage Research, Institute of Metabolism and Systems Research, University of Birmingham, Birmingham
2. Birmingham Women's and Children's NHS Foundation Trust, Mindelsohn Way, Birmingham
3. Spanish Council for Scientific Research, Institute of Environmental Assessment and Water Research, Barcelona, Spain
4. Heartlands Hospital, Heart of England NHS Foundation Trust, Bordesley Green East, Birmingham
5. Reproductive health, Warwick University, Coventry.