video: In this movie, which plays 360-times faster than real time, white spots represent light emitted from single fluorescent spliceosome subunits as they interact with single RNA (ribonucleic acid) molecules attached to a glass surface. This movie shows that in the presence of a common source of cellular energy, ATP (adenosine-5'-triphosphate), the U1, U2, U5 and NTC subunits of the spliceosome can stably interact with the RNA and generate white spots that last for periods of time. This video relates to an article that appeared in the March 11, 2011, issue of Science, published by AAAS. The study, by Dr. Aaron A. Hoskins, at Howard Hughes Medical Institute in Worcester, Mass., and colleagues was titled, "Ordered and Dynamic Assembly of Single Spliceosomes." view more
Credit: Video file courtesy of Aaron A. Hoskins, Jeff Gelles and Melissa J. Moore