Foot-and-mouth disease virus (FMDV), Classical swine fever virus (CSFV) and Swine vesicular disease virus (SVDV) all cause important veterinary diseases. Handling of these viruses, in particular FMDV and CSFV, is only allowed within high containment laboratories and stables. Occasionally such facilities need to be decommissioned for repair or when closing down, which is normally done by fumigation. However, building materials in older laboratories or stables that have housed infected animals may not be well-suited for fumigation. Heat treatment may serve as another way to decommission such facilities. While heat-inactivation of different viruses in different solutions has been investigated earlier in several studies, however, knowledge about heat-inactivation of viruses when they are in an air-dried form has been limited.
In this study, the authors of this article inactivated the virus to below the limit of detection of the assay. The heated samples were compared with samples incubated at room temperature (RT). All of the tested viruses, including multiple FMDV strains, SVDV and CSFV, having been air-dried onto surfaces, were shown to be inactivated after treatment at 70 °C for 2 days; while most of these viruses survived for more than 2 days at RT. The data obtained from this study clearly demonstrate that heating is an effective way of inactivating various viruses that have been air-dried onto either a plastic or a glass surface. The findings are important in relation to decommissioning of laboratories where virus work has been carried out or for stables that have housed infected animals, where the buildings are not well-suited for fumigation.
Keywords: Virus survival, Picornavirus, Pestivirus, Decontamination, Risk-assessment
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