News Release

Heat inactivation of foot-and-mouth disease virus, swine vesicular disease virus and classical swine fever virus

Peer-Reviewed Publication

Compuscript Ltd

Figure 1

image: Inactivation of different FMDV strains by heat. Aliquots of the different FMDV stocks were air-dried in the biosafety cabinet (BSC) for 1 h on either glass (left panels) or plastic (right panels) (Day 0). The dried virus samples were incubated for 1, 2, 3, 5 or 7 days at either RT (blue line) or 70 °C (red line). Virus strains assayed were: (A) FMDV A10 Holland on glass, (B) FMDV A10 Holland on plastic, (C) FMDV A Iraq on glass, (D) FMDV A Iraq on plastic, (E) FMDV Asia-1 Shamir on glass, (F) FMDV Asia-1 Shamir on plastic, (G) FMDV O1 Lausanne on glass and (H) FMDV O1 Lausanne on plastic. If no virus was detected in any of the five wells, the titer is ≤ 101.8 TCID50/ml, which is indicated by the dotted line. view more 

Credit: Biosafety and Health

Foot-and-mouth disease virus (FMDV), Classical swine fever virus (CSFV) and Swine vesicular disease virus (SVDV) all cause important veterinary diseases. Handling of these viruses, in particular FMDV and CSFV, is only allowed within high containment laboratories and stables. Occasionally such facilities need to be decommissioned for repair or when closing down, which is normally done by fumigation. However, building materials in older laboratories or stables that have housed infected animals may not be well-suited for fumigation. Heat treatment may serve as another way to decommission such facilities. While heat-inactivation of different viruses in different solutions has been investigated earlier in several studies, however, knowledge about heat-inactivation of viruses when they are in an air-dried form has been limited.

In this study, the authors of this article inactivated the virus to below the limit of detection of the assay. The heated samples were compared with samples incubated at room temperature (RT). All of the tested viruses, including multiple FMDV strains, SVDV and CSFV, having been air-dried onto surfaces, were shown to be inactivated after treatment at 70 °C for 2 days; while most of these viruses survived for more than 2 days at RT. The data obtained from this study clearly demonstrate that heating is an effective way of inactivating various viruses that have been air-dried onto either a plastic or a glass surface. The findings are important in relation to decommissioning of laboratories where virus work has been carried out or for stables that have housed infected animals, where the buildings are not well-suited for fumigation.


Keywords: Virus survival, Picornavirus, Pestivirus, Decontamination, Risk-assessment

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CiteScore: 3.8


ISSN 2590-0536

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