News Release

Crop protection: Biohacking against fungal attacks

KIT and research partners develop sustainable crop protection solutions

Business Announcement

Karlsruher Institut für Technologie (KIT)

The DialogProTec research project developed sophisticated technologies for sustainable plant protection

image: The DialogProTec research project developed sophisticated technologies for sustainable plant protection (montage: DialogProTec, KIT). view more 

Credit: montage: DialogProTec, KIT

Harmful fungi cause enormous agricultural losses. Conventional techniques for combating them involve the use of poisonous fungicides. Researchers at Karlsruhe Institute of Technology (KIT), working with partners from Germany, France, and Switzerland on the DialogProTec project, have developed environmentally safe alternatives that trick the pathogens’ chemical communication with plants. Now that the research has been completed, the new technology is ready for use.

First, the leaves turn brown, then the entire grapevine dies. A fungal infection called esca is a threat to wine production in Europe and causes millions in damage to winemakers every year. “This disease has been known in Southern Europe since the Middle Ages, but it never played a major role,” says Dr. Alexandra Wolf from KIT’s Botanical Institute, which coordinates the DialogProTec project. “But because of climate change, the fungus is now encountering many plants weakened by climate stress.” Conventional plant protection usually involves the use of poisonous fungicides to fight fungal diseases like esca. In DialogProTec, the researchers have developed a completely new approach that works without any environmentally hazardous toxins. “In nature, organisms interact using chemical signals. We’ve been able to identify some of the signals between the host and the pathogen, and to manipulate them,” says Wolf, who adds that this “biohack” is precise and effective and has a minimal ecological footprint.

To develop the new methods, the KIT-led project founded an interdisciplinary research network including specialists in botany, fungal genetics, microsystem technology, organic chemistry, and agricultural sciences. The network used about 20,000 fungus strains from the collection at the Institute of Biotechnology and Drug Research (IBFW) in Kaiserslautern and about 6,000 plant species from KIT.

Tracking down Signal Substances with High Tech

The researchers didn’t need to work with entire plants and fungi to identify and exploit the right signals. Instead, they worked with individual cells. A microfluidics chip jointly developed with KIT’s Institute of Microstructure Technology served as the basis for a miniature ecosystem. “We placed plant and fungi cells on chips a few square centimeters in size so that they can’t come into physical contact but can interact chemically via a microfluidic current,” says Christian Metzger from the Botanical Institute at KIT. “To make this interaction visible, we equipped the genetic material in the plant cells with a gene switch and a fluorescence gene. Whenever a chemical signal activates the immune system, we can measure the green fluorescence.” The gene switches are from wild grapevines, in which the researchers had previously detected an especially active immune response.

Plant Vaccination Ready to Test

During their investigations, the researchers first decoded the chemical communication between fungus and plant that accompanies a fungal attack. One of the things they identified was signal substances that the fungus uses to suppress the plant’s immune response. “They’re part of a chemical interaction shaped by a long evolutionary process and are produced as soon as the fungus detects specific stress signals from the plant,” explains Professor Peter Nick, who heads the project and the Botanical Institute. The team then identified molecules that could be used to reactivate the immune response. “When we use them for plant protection, the plants can often ward off the fungus. You can think of it as a vaccination for plants,” says Nick.

DialogProTec’s innovative technology is already on its way into practical use and is soon to be tested in the field. In addition to their work on an alternative to fungicides, the project team has also developed new approaches to promoting plant growth or fighting weeds, where signal substances could also replace poisonous herbicides in the future.

About DialogProTec

In addition to KIT, the University of Freiburg, the University of Strasbourg, the Institute of Biotechnology and Drug Research (IBFW), and the Research Institute of Organic Agriculture (FiBL) in Switzerland were also involved in the research, which was funded by the “Interreg Oberrhein” cross-border EU program. Over the course of the project, a network of agricultural operations (including wine and fruit producers) and industrial companies (plant protection and technology businesses) formed; the researchers will continue to cooperate with it in the future.

More information: https://www.dialogprotec.eu/start_en.htm 

More information on the KIT Climate and Environment Center: https://www.klima-umwelt.kit.edu/english/index.php

Being “The Research University in the Helmholtz Association”, KIT creates and imparts knowledge for the society and the environment. It is the objective to make significant contributions to the global challenges in the fields of energy, mobility, and information. For this, about 9,800 employees cooperate in a broad range of disciplines in natural sciences, engineering sciences, economics, and the humanities and social sciences. KIT prepares its 22,300 students for responsible tasks in society, industry, and science by offering research-based study programs. Innovation efforts at KIT build a bridge between important scientific findings and their application for the benefit of society, economic prosperity, and the preservation of our natural basis of life. KIT is one of the German universities of excellence.


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