National media relations manager
TAMPA, Fla. (Oct. 6, 2022) – Researchers at the USF Health Morsani College of Medicine, located at the University of South Florida, successfully tested a protein that has the potential to aid in the development of a protein-based therapy for patients with ALS, a progressive nervous system disease, also known as Lou Gehrig’s disease, that affects nerve cells in the brain and spinal cord.
Published in eNeuro, the study examines the effects of apolipoprotein A1, a “good cholesterol” on endothelial cells, the lining in blood vessels that provides a barrier between the brain, spinal cord tissues and blood circulation.
In a petri dish under an environmental condition reminiscent of ALS, the team found that the protein activates a unique pathway inside cells that increases survival and protects endothelial cells from toxic substances in the blood. This pathway can enhance the survival of cells and prevent further vascular damage by ALS.
“With a functional barrier, the hope is that the environment in the central nervous system will become less toxic and disease progression can be slowed,” said Svitlana Garbuzova-Davis, professor at the Department of Neurosurgery and Brain Repair and lead investigator.
While the protein has proven to protect endothelial cells in diseases such as diabetes and atherosclerosis, the effects on ALS-damaged endothelial cells were previously unknown.
To test the impact on ALS, the team examined how the protein affects endothelial cell signals. Garbuzova-Davis and co-investigators USF Professor Alison Willing and USF Distinguished University Professor Cesario Borlongan discovered that the injured cells absorb the protein, significantly reducing endothelial cell damage.
“It’s a little early to predict what the ultimate effect is for patients,” Willing said. “In this study, we used a cell culture model where we can control all aspects of what the cells are exposed to. It is not possible to have the same degree of control in people.”
Garbuzova-Davis says this study laid the groundwork for additional research with animal models of ALS to fully determine the therapeutic effects of the protein. Upon successful results, apolipoprotein A1 may be considered for clinical trials, where it would be evaluated for safety and efficacy in ALS patients.
In the near future, apolipoprotein A1 may be considered a potential novel therapeutic for endothelial cell repair, restoring the central nervous system barrier in ALS patients. It may also help alleviate ALS symptoms, such as imbalanced antioxidant levels and inflammation.
About the University of South Florida
The University of South Florida, a high-impact global research university dedicated to student success, generates an annual economic impact of more than $6 billion. Over the past 10 years, no other public university in the country has risen faster in U.S. News and World Report’s national university rankings than USF. Serving more than 50,000 students on campuses in Tampa, St. Petersburg and Sarasota-Manatee, USF is designated as a Preeminent State Research University by the Florida Board of Governors, placing it in the most elite category among the state’s 12 public universities. USF has earned widespread national recognition for its success graduating under-represented minority and limited-income students at rates equal to or higher than white and higher income students. USF is a member of the American Athletic Conference. Learn more at www.usf.edu.
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Apolipoprotein A1 Enhances Endothelial Cell Survival in an In Vitro Model of ALS
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