News Release

New strategy for cryopreservation of mouse follicles

Peer-Reviewed Publication

University of Science and Technology of China

Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

image: Schematic of microencapsulation, cryopreservation, in vitro development and fertilization of PAFs. view more 

Credit: Image by TIAN Conghui et al.

Recently, a research team led by Prof. ZHAO Gang, Prof. SHI Qinghua from the University of Science and Technology (USTC), and Prof. CAO Yunxia from Anhui Medical University, realized the high-quality cryopreservation of mouse preantral follicles (PAFs) based on biomimetics and physical field-assisted ice inhibition. This work was published in Nature Communications.

Fertility preservation is the only method to overcome the decrease in female fertility, a major health issue of common concern. The cryopreservation of follicles plays an important role in fertility preservation because follicles are rich in resources, easy to obtain and do not involve in ethical issues. However, the existing methods for follicles cryopreservation are complex and ineffective due to the high concentration of toxic cryoprotective agents.

To solve this problem, the research team developed the low-concentration cryoprotective agent vitrification cryopreservation of mouse PAFs based on active ice inhibition approach. They combined physical field-assisted space heating techniques such as electromagnetic and laser heating with hydrogel microencapsulation, thus reducing the concentration of penetrating cryoprotective agents by 75%.

The physical field-assisted space heating techniques effectively improve the speed of heating and the uniformity of temperature distribution, suppressing the possible devitrification or recrystallization that could cause fatal damage to cells during the warming process. Hydrogel microencapsulation provides biomimetic cryopreservation and critical biochemical and mechanical microenvironment, reducing the demand for toxic cryoprotective agents and supporting the in vitro development of follicles. It also isolates the follicles from external magnetothermal and photothermal materials, which improves biosecurity.

The results showed that the survival rate of follicles preserved by this method was improved by about 1/3. Furthermore, the team managed to obtain oocytes after 3D in vitro culture of the cryopreserved PAFs. The oocytes were then fertilized in vitro and transplanted into surrogate mice, which gave birth to healthy offspring.

This research built a platform that integrated microencapsulation, cryopreservation and 3D in vitro culture, providing a unique solution for the preservation and utilization of follicles.

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