News Release

Oxygen groups key to unlocking graphene’s antimicrobial potential

Peer-Reviewed Publication

University of Birmingham

The amount of surface oxygen in graphene materials is a key factor in how effective they could be in killing bacteria – a discovery which may help to design safer and more effective products to combat antimicrobial resistance.

Graphene oxide with high surface oxygen content (SOC) is very flexible and can wrap around bacteria (a parallel mode of contact), but when it has lower SOC the material has higher rigidity and tends to contact bacteria edgewise (in a perpendicular mode).

Neither mode necessarily kills bacteria, but bactericidal activity depends on how the material interacts with surrounding biomolecules. The discovery will help scientists to understand the different possible physical mechanisms leading to their antibacterial activity.

Publishing their findings in ACS nano, an international group of scientists from the UK, Cyprus, Austria, Finland, The Netherlands and China reveal that it is graphene oxide’s different interaction modes that lead to distinct antibacterial activity – with a ‘switch’ occurring when surface oxygen levels reach a certain  threshold.

A slight change of SOC can lead to the shift of interaction modes between parallel and perpendicular contact. “The impact of SOC on the interaction mode has been underestimated for a long time”, commented Dr Zhiling Guo, from the University of Birmingham.

Dr. Peng Zhang, from the University of Birmingham, commented: “Our research highlights that surface oxygen levels can help to evaluate the antibacterial effects of graphene materials - helping to design safer materials through clarifying the role of SOC.”

As antimicrobials, graphene materials may have advantages over traditional antibiotics due to their physical mechanisms of action which ensure less chance of development of microbial resistance.

Until now, the fundamental question as to whether the antibacterial mechanism of graphene materials originates from parallel interaction or perpendicular interaction, or from a combination of these, remains poorly understood - hindering progress in developing antibacterial graphene materials and understanding their environmental safety.

Professor Iseult Lynch, from the UnIversity of Birmingham noted that “The discovery is a potential ‘gamechanger’ and we should be using this surface oxygen ‘switch’ as the determining property to define and classify graphene materials in the context of human health and environmental safety.”

The UK- led international research team created a series of graphene materials with different SOCs and compared their antibacterial performance - evaluating total cell growth, biofilm formation and oxidative stress, as well as physical interactions with bacteria including through molecular simulations.

Different interaction modes lead to distinct antibacterial activity and interaction mode is highly related to the rigidity of the graphene materials which depends on the amount of surface oxygen.

The antibacterial activity of graphene materials was reported as early as 2010. The material has been used to create antibacterial fabrics for maternity garments which can prevent microbial growth on the fabric surface. Graphene-coated nonwoven fabrics have been used to produce antibacterial masks, while graphene-based membranes have been extensively studied for water treatment because of their ultrafast water transport and antifouling activity.


For more information, please contact Tony Moran, International Communications Manager, University of Birmingham on +44 (0)782 783 2312 or For out-of-hours enquiries, please call +44 (0) 121 414 2772.

Note for Editors

  • The University of Birmingham is ranked amongst the world’s top 100 institutions, its work brings people from across the world to Birmingham, including researchers and teachers and more than 8,000 international students from over 150 countries.
  • Defining the Surface Oxygen Threshold That Switches the Interaction Mode of Graphene Oxide with Bacteria’ - Zhiling Guo, Peng Zhang, Changjian Xie, Evangelos Voyiatzis, Klaus Faserl, Andrew J. Chetwynd, Fazel Abdolahpur Monikh, Georgia Melagraki, Zhiyong Zhang, Willie J. G. M. Peijnenburg, Antreas Afantitis, Chunying Chen, and Iseult Lynch is published by ACSnano.
  • Participating institutions include the University of Birmingham, UK;  Institute of Medical Biochemistry, Medical University of Innsbruck, 6020 Innsbruck, Austria; Institute of Environmental Sciences (CML), Leiden University, Einsteinweg 2, 2333 CC Leiden, The Netherlands; Department of Environmental & Biological Sciences, University of Eastern Finland, P.O. Box 111, Joensuu, FI-80101, Finland;  NovaMechanics Ltd., Cyprus ;Chinese Academy of Science (CAS) Center for Excellence in Nanoscience and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Beijing; Research Unit of Nanoscience and Technology, Chinese Academy of Medical Sciences, Beijing; GBA National Institute for Nanotechnology Innovation, Guangdong, China.

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