News Release

CHOP researchers reveal complex assembly process involved in DNA virus replication

Study shows viral proteins utilize phase separation to coordinate complex process of replicating viral genomes and then encapsulating that DNA in a viral particle

Peer-Reviewed Publication

Children's Hospital of Philadelphia

Philadelphia, April 5, 2023—In a twist on the question, “Which came first, the chicken or the egg?”, scientists have long faced a similar question about how human adenovirus replicates: “Which comes first, assembly of the viral particle, or packaging of the viral genome?” Now, in a new study published today in Nature, researchers at Children’s Hospital of Philadelphia (CHOP) have answered that question, showing that viral proteins use a process called phase separation to coordinate production of viral progeny. 

“This study answers a fundamentally important question: how a viral nucleic acid gets inside a particle so that viral offspring can be delivered to cells,” said Matthew Charman, PhD, a research associate in the Weitzman Lab at Children’s Hospital of Philadelphia. “These findings have broad implications, from potential therapeutic interventions to improved gene therapy delivery, in addition to expanding our understanding of basic cell biology.”

Viruses hijack host cellular processes to replicate and produce infectious offspring that are key for viral spread and transmission. To do so, they must both replicate their viral genomes and package those genomes into viral particles, so that the infectious cycle can continue. However, little is known about how genome replication, particle assembly, and genome packaging are coordinated in the crowded nuclear environment. 

“If we think of viral replication as an old-fashioned milk assembly line, we know how the milk bottles are formed and that they come out filled, but prior to this study, the process of filling them was somewhat of a black box,” said senior author Matthew D. Weitzman, PhD, a professor in CHOP’s Department of Pathology and Laboratory Medicine. “Our findings suggest that the viral particle forms around the viral genome. Extending the analogy, many have assumed that the bottle must be made before being filled, but it turns out the bottle is actually formed around the milk. Led by Dr. Charman, we have shown that a biophysical process known as phase separation allows this process to occur in an orderly, coordinated fashion.”

Emerging evidence suggests that membraneless compartments form inside virus-infected cells by phase separation. These membraneless compartments, known as biomolecular condensates (BMCs), can regulate biological processes by concentrating or sequestering biomolecules in an enriched dense phase, while limiting their concentration in the light phase. Although BMCs have been linked to several viral processes, there was insufficient evidence that phase separation contributes functionally to the assembly of infectious viral offspring in infected cells. 

To investigate the potential role of BMCs in this process, the researchers studied adenovirus, a nuclear-replicating DNA virus. Because the adenovirus proteins involved in genome replication are distinct from those involved in particle assembly and genome packaging, the researchers reasoned focusing on this virus would allow them to dissect and more easily identify the role of phase separation in specific viral processes.

Through a variety of techniques, including homopropargylglycine (HPG) labeling and fluorophore click chemistry, the researchers demonstrated that the adenovirus 52 kDa protein – a dedicated assembly/packaging protein – makes its own membraneless structures through phase separation and plays a critical role in the coordinated assembly of new infectious particles. They showed that not only does the 52 kDa protein organize viral capsid proteins into nuclear BMCs, but also that this organization is essential for the assembly of complete, packaged particles containing viral genomes.

Additionally, the researchers performed experiments with a mutant adenovirus lacking the 52 kDa protein and showed that incomplete capsids formed in the absence of viral BMCs. Thus, the researchers were able to show that by altering the formation of these membraneless structures within the cell, the “assembly line” producing viral offspring no longer functioned properly.

“Now knowing these steps, the question becomes: could we reengineer viruses based on this biological process to, for example, become better delivery vehicles for innovations like gene therapy?” Dr. Charman said. “Understanding how viruses are made opens up a world where we could not only potentially target those viruses more effectively in the future but also create gene therapy tools that lack the limitations of current delivery approaches.”

This research was supported by NIAID grants R01-AI145266 and R01-AI121321. 

Charman et al. “A viral biomolecular condensate coordinates assembly of progeny particles,” Nature, April 5, 2023, DOI: 10.1038/s41586-023-05887-y


About Children’s Hospital of Philadelphia: A non-profit, charitable organization, Children’s Hospital of Philadelphia was founded in 1855 as the nation’s first pediatric hospital. Through its long-standing commitment to providing exceptional patient care, training new generations of pediatric healthcare professionals, and pioneering major research initiatives, the 595-bed hospital has fostered many discoveries that have benefited children worldwide. Its pediatric research program is among the largest in the country. The institution has a well-established history of providing advanced pediatric care close to home through its CHOP Care Network, which includes more than 50 primary care practices, specialty care and surgical centers, urgent care centers, and community hospital alliances throughout Pennsylvania and New Jersey, as well as an inpatient hospital with a dedicated pediatric emergency department in King of Prussia. In addition, its unique family-centered care and public service programs have brought Children’s Hospital of Philadelphia recognition as a leading advocate for children and adolescents. For more information, visit 


Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.