News Release

A novel function of the ventral lateral preoptic area of the hypothalamus in inducing arousal

Peer-Reviewed Publication

University of Tsukuba

Tsukuba, Japan—Deep within the intricate realms of the brain, a crucial component called the hypothalamus serves as a vital regulator of homeostasis. Within this remarkable structure, two distinct regions are found to be responsible for sleep and wakefulness: the preoptic area (POA) and the lateral hypothalamus (LHA), respectively. Specifically, the ventral lateral preoptic area (VLPO) in the preoptic area houses neurons known as VLPOGABA and VLPOGAL neurons, which produce gamma-aminobutyric acid and galanin (GAL) to promote sleep. Alternatively, the LHA harbors orexin neurons that play a crucial role in sustaining wakefulness. Together, these interconnected regions within the hypothalamus delicately balance restful slumber and vigilant wakefulness.

In our study using mice, we made the following intriguing discovery: VLPOGABA and VLPOGAL neurons form direct connections, or synapses, with orexin neurons in the LHA. Additionally, we identified numerous groups of neurons that project to VLPOGABA and VLPOGAL neurons and are thought to regulate their activity. Notably, these neuron groups were most prominent in the preoptic area and LHA. Furthermore, when we artificially stimulated only the neural circuits connecting VLPOGABA neurons to the LHA, we observed the induction of arousal instead of sleep, challenging previous assumptions. Our study sheds light on the role of the coupling between VLPO and LHA in regulating sleep and wakefulness, unveiling a previously unknown function of the neural circuit connecting these two regions in controlling wakefulness.

This study was supported by a JSPS KAKENHI Grant-in-Aid for Scientific Research (B) (JP 18H02595) (TS); AMED Grant Number JP21zf0127005 (TS); and JST CREST Grant Number JPMJCR1655 Japan (TS); TMFC Japan Foundation for applied Enzymology (AH).

Original Paper

Title of original paper:
Structure and Function of Neuronal Circuits linking Ventrolateral Preoptic Nucleus and Lateral Hypothalamic Area

The Journal of Neuroscience



Professor SAKURAI Takeshi
Assistant Professor HIRANO Arisa
International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba / Institute of Medicine, University of Tsukuba

Related Link

International Institute for Integrative Sleep Medicine (WPI-IIIS)
Institute of Medicine

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