One of the more unusual ways objects can increase longevity is by sacrificing a part of themselves: From dummy burial chambers used to deceive tomb raiders, to a fuse melting in an electrical circuit to safeguard appliances, to a lizard’s tail breaking off to enable its escape. Sacrificial parts can also be found within collagen, the most abundant protein in our bodies. Scientists at the Heidelberg Institute for Theoretical Studies (HITS) have revealed how the rupture of weak sacrificial bonds within collagen tissue helps to localize damage caused by excessive force, minimize negative impacts on the wider tissue, and promote recovery. Published in Nature Communications, the work shines light on collagen’s rupture mechanisms, which is crucial for understanding tissue degradation, material ageing, and potentially advancing tissue engineering techniques.
“Collagen’s remarkable crosslink chemistry appears to be perfectly adapted to handling mechanical stress,” says Frauke Gräter, who led the research at HITS. “By using complementary computational and experimental techniques to study collagen in rat tissue, our findings indicate that weak bonds within the crosslinks of collagen have a strong propensity to rupture before other bonds, such as those in the collagen's backbone. This serves as a protective mechanism, localizes the detrimental chemical and physical effects of radicals caused by ruptures, and likely supports molecular recovery processes.”
Collagen comprises roughly 30 percent of all proteins in the human body. It provides strength to bones, elasticity to skin, protection to organs, flexibility to tendons, aids in blood clotting, and supports the growth of new cells. Structurally, collagen resembles a triple-braided helix: Three chains of amino acids intertwine to form a strong and rigid backbone. Each collagen fibre contains thousands of individual molecules that are staggered and bound to each other by crosslinks, contributing to collagen's mechanical stability. It was thought that collagen crosslinks are susceptible to rupture, however little was known about the specific sites of bond ruptures or why ruptures occur where they do.
Scientists from the Molecular Biomechanics Group at HITS aimed to unravel these puzzles using computer simulations of collagen across multiple biological scales and under different mechanical forces. They validated their findings via gel electrophoresis and mass spectrometry experiments conducted on rat tails, flexors, and Achilles tendons. By subjecting collagen to rigorous testing, the team was able to determine specific breakage points. They observed how force dissipates through the complex hierarchical structure of the tissue and how its chemical bonds bare the load.
Mature crosslinks in collagen consist of two arms: one of which is weaker than other bonds in collagen tissue. When subjected to excessive force, the weaker arm is typically first to rupture, dissipating the force and localizing detrimental effects. The scientists found that in regions of collagen tissue where weak bonds are present, other bonds – both in the crosslinks and the collagen backbone – are more likely to remain intact, thereby preserving the structural integrity of the collagen tissue.
Previous work led by HITS scientists revealed that excessive mechanical stress on collagen leads to the generation of radicals, which in turn cause damage and oxidative stress in the body. ”Our latest research shows that sacrificial bonds in collagen serve a vital role in maintaining the overall integrity of the material can help to localize the impacts of this mechanical stress that could otherwise have catastrophic consequences for the tissue”, explains Benedikt Rennekamp, the study's first author. “As collagen is a major substituent of tissues in our bodies, by uncovering and understanding these rupture sites, researchers can gain valuable insights into the mechanics of collagen and potentially develop strategies to enhance its resilience and mitigate damage.”
Rennekamp, B., Karfusehr, C., Kurth, M. et al. Collagen breaks at weak sacrificial bonds taming its mechanoradicals. Nat Commun 14, 2075 (2023). https://doi.org/10.1038/s41467-023-37726-z
Dr. Peter Saueressig
Head of Communications
Heidelberg Institute for Theoretical Studies (HITS)
Tel: +49 6221 533 245
Prof. Dr. Frauke Gräter
Group Leader Molecular Biomechanics (MBM)
Heidelberg Institute for Theoretical Studies (HITS)
Phone: +49 6221 533 267
HITS, the Heidelberg Institute for Theoretical Studies, was established in 2010 by physicist and SAP co-founder Klaus Tschira (1940-2015) and the Klaus Tschira Foundation as a private, non-profit research institute. HITS conducts basic research in the natural, mathematical, and computer sciences. Major research directions include complex simulations across scales, making sense of data, and enabling science via computational research. Application areas range from molecular biology to astrophysics. An essential characteristic of the Institute is interdisciplinarity, implemented in numerous cross-group and cross-disciplinary projects. The base funding of HITS is provided by the Klaus Tschira Foundation.
Method of Research
Subject of Research
Human tissue samples
Collagen breaks at weak sacrificial bonds taming its mechanoradicals.
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