News Release

Spatial dimension of macrophage heterogeneity in liver diseases

Peer-Reviewed Publication

First Hospital of Jilin University

Recent technological breakthroughs greatly advanced our understanding of liver macrophage spatiotemporal diversity.

image: For instance, single-cell suspensions subjected to single-cell transcriptome analysis revealed the simultaneous presence of multiple immune cell phenotypes. Multiplex immunohistochemistry and imaging mass cytometry showed complex and spatially restricted accumulation of defined monocytic populations. In vivo time-lapse microscopy unravelled the timing of the immune response and dynamic macrophage functionality. Organ clearing and three-dimensional imaging further deepened in situ macrophage biology understanding. view more 

Credit: By Adrien Guillot & Frank Tacke

This review manuscript highlights the relevance of spatially-resolved macrophage phenotyping in liver disease-related research.

The liver is a vital organ heavily populated with macrophages, which represent key players of the innate immune response but also hold key functions in the maintenance of a healthy organ. Liver macrophages are mostly distributed across two populations of different origins and functions during homeostasis and disease: liver resident macrophages (referred to as Kupffer cells, KCs), and monocytic macrophages derived from the bone-marrow (MoMFs). The KC: MoMF ratio, as well as their respective spatial distribution through the liver, are increasingly recognized as liver disease progression hallmarks. These phenomena are observed in a wide variety of liver diseases ranging from non-alcoholic fatty liver disease to biliary tree disorders (cholangiopathies).

Recent single cell analyses revealed previously underestimated heterogeneities within those two major populations. These studies were rendered possible by recent technological breakthroughs in transcriptomics, proteomics and bioinformatics, particularly in single cell RNA sequencing and multiplex immunostaining. Nowadays, the map of the liver macrophage landscape is being refined to include this phenotypical and functional heterogeneity, in a 2- and 3-dimensional resolved manner.

The most striking changes occurring at later stages of liver disease include a depletion in KCs and an accumulation of MoMFs with a strong pro-inflammatory phenotype and poor phagocytosis capacities in perilobular fibrotic areas, while lipid-associated macrophages are observed in steatotic areas during fatty liver disease.


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