Microbes, thriving in even the world's most extreme environments, are capable of performing myriad biological functions, learned over the billions of years they have inhabited the planet. On May 23, at the general meeting of the American Society of Microbiology (ASM), the U.S. Department of Energy Joint Genome Institute (DOE JGI) will announce that it has finished the sequence of100 microbial genomes and released this information for the benefit of the global research community.