Propelling Biomaterial Innovations for Advanced Cell Therapy (IMAGE)
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In vitro evaluation of osteogenic differentiation of rMSCs on different material formulations after 0, 3, 7 and 14 days of incubation. (A) ALP assay evaluation: (Ai) representative microscopic images of ALP staining and (Aii) quantitative analysis of ALP activity. (B) ARS assay evaluation: (Bi) representative microscopic images of ARS staining and (Bii) quantitative analysis of matrix mineralization by measuring the absorbance of the extracted solution at a wavelength of 592 nm with a microplate reader. qRT-PCR analysis of (C) osteogenesis-related gene expressions including (Ci) OCN, (Cii) Col-1, (Ciii) Runx2 and (Civ) ALP, and (D) mechanosensing-related gene expressions including (Di) integrin α2, (Dii) FAK, (Diii) ERK1 and (Div) ERK2 in rMSCs after culture on different material surfaces for 7 days. Data are presented as mean ± SD and analyzed by one-way ANOVA (n = 3 for each group, *p < 0.05).
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