Figure 3 (IMAGE)

Institute for Basic Science

Figure 3

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Investigation of the LDs with time. Two-color (excitation: 2,193 cm-1 and 2,855 cm-1) IP images of fixed U2OS cells were measured at different time points after PA-d31 administration. PA-d31 treatment times were indicated in the upper left corner of each image. The color scheme is the same as in Figure 2A. Each scale bar indicates a length of 20 μm. Corresponding IP spectra of LDs were also measured. The y-axis scales in the two regions (2,030 cm-1 to 2,230 cm-1 and 2,750 cm-1 to 2,950 cm-1) are adjusted differently to enhance clarity.

The images and corresponding spectra demonstrate the capability of 2C-IPM to analyze the mole fractions of inherent and newly synthesized neutral lipids in time.

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Institute for Basic Science

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