Figure 2 (IMAGE)

Institute for Basic Science

Figure 2

Caption

Two-color infrared photothermal imaging (IPI) measurements of U2OS cells cultured and exposed to palmitic acid (PA) and its deuterated analog (PA-d31). (A) Two-color IP images of fixed U2OS cells cultured in different growth media (250 µM PA, 250 µM PA-d31, and standard medium) for 24 hours. The green and red false colors indicate IPI contrasts of 2,855 cm-1 and 2,193 cm-1, respectively. Each scale bar indicates a length of 20 μm. (B) Representative IP spectra of LDs are observed in Figure 3A at two different spectral regions.

The images and corresponding spectra demonstrate the capability of 2C-IPM to measure the spatial distribution of LDs and distinguish inherent and newly synthesized neutral lipids.

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Institute for Basic Science

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