Composition of cell types in healthy mouse liver. (IMAGE)

First Hospital of Jilin University

Composition of cell types in healthy mouse liver.

Caption

(A and B) Formalin-fixed, paraffin-embedded liver sections from 8~10 weeks old C57BL/6J mice were sequentially stained with markers as indicated. The upper panel shows the representative staining of portal vein region (1), mid zone region (2) and central vein region (3). The lower panel shows nuclei segmentation performed using ilastik and CellProfiler. Each type of cell nuclei was labelled with colour as indicated. (C) Summary of percentage of each cell type. Value represents the average of six mice. BDC, bile duct cell; CV, central vein; DAPI, 4',6-diamidino-2-phenylindole; Hep, hepatocytes; HNF, hepatocyte nuclear factor; HSC, hepatic stellate cell; IBA1, ionized calcium-binding adaptor molecule 1; LSEC, liver sinusoidal endothelial cell; MAIT, mucosal-associated invariant T; Neu, Neutrophil; NK, natural killer; PV, portal vein.

Credit

By Dechun Feng, Yukun Guan, Yang Wang, Luca Maccioni, Bryan Mackowiak, Bin Gao

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License

CC BY-NC

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