APPswe affects the osteogenic differentiation and proliferation of MSCs by regulating the Notch signaling. (IMAGE)
Caption
(A) The mRNA expression levels of Hey1 and Hes1 in each treatment group were measured by quantitative real-time PCR. (B) Western blot analysis of NICD expression in the cytoplasm and nucleus in each treatment group. (C) The expression of NICD in each treatment group was detected by cellular immunofluorescence. (D, E) ALP staining and activity detection were performed in each treatment group. (F, G) The formation of calcium nodules in each treatment group was analyzed by ARS staining and semiquantitative analysis. MSCs were treated with APPswe or APPswe-C for 24 h and then with BMP2 for 48 h to detect the expression levels of related proteins. n = 3; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. APPswe, Swedish mutant amyloid precursor protein; APPswe-C, APPswe without an intracellular segment; MSC, mesenchymal stem cell; ALP, alkaline phosphatase; BMP2, bone morphogenetic protein 2; NICD, the Notch intracellular domain.
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Genes & Diseases
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