Effects of APPswe on BMP2-induced bone formation and repair in rBMSCs in vivo. (IMAGE)
Caption
(A) Six weeks after surgery, the rat skulls were recovered for micro-CT analysis, and representative images were shown. (B–G) Histomorphometric analysis of structural bone parameters. Bone volume (BV, mm3), relative bone volume (BV/total volume, %), trabecular number (Tb.N, 1/mm), trabecular separation (Tb.Sp, mm), bone mineral density (BMD, mg HA/ccm), and trabecular thickness (Tb.Th, mm) were calculated based on the micro-CT scanning data. (H) Representative hematoxylin-eosin staining, Masson's trichrome staining, and Alcian blue staining of each group are shown. (I) The expression levels of ALP, collagen I, and NICD were confirmed by immunohistochemical staining. Representative images of each group are shown. The red line indicates the location and width of the skull defect in each group. The black box in the tissue staining image is the locally representative region of the lower-magnification image. The black arrows indicate positive protein expression in new bone. n = 3; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. APPswe, Swedish mutant amyloid precursor protein; rBMSC, rat bone mesenchymal stem cell; ALP, alkaline phosphatase; NICD, the Notch intracellular domain; NB, new bone; HB, host bone.
Credit
Genes & Diseases
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